OVARIAN CATEGORIES, FOLLICLES AND OOCYTES ANALYSIS OF BUFFALO IN VIEW OF IN VITRO PRODUCTION OF EMBRYOS

Abstract

The present study was carried out at the Department of Animal Nutrition, Genetics and Breeding, Sher-e-Bangla Agricultural University, Dhaka during the period from July 2018 to June 2019. Buffalo ovaries were collected from Slaughter house of Dhaka city. During collection left and right ovaries were identified and recorded and after necessary processing the ovaries were categorized as (i) ovaries without corpus luteum (CL) and (ii) ovaries with corpus luteum (CL). Ovaries were then evaluated on the basis of length (cm), width (cm), weight (g), total number of follicles on the surface of each category ovaries, number of follicles aspirated and total number of COCs. In terms of ovarian categories regarding left and right category, the result obtained from this experiment showed significant (p<0.01) difference in the parameters. The highest percentage of CL (27.12±0.11%) was in right ovary compared to left ovaries (3.24±0.11%). The higher length (cm) (2.75±0.06), width (cm) (2.17±0.05), weight (g) (4.74±0.13) was found in right ovaries compared to left ovaries. The number of follicles (aspirated) (5.47±0.16) were also higher in left ovaries compared to right ovaries (4.18±0.16). The normal COCs were higher in case of left ovaries (with a mean of 0.98±0.16 per ovary) compared to right ovaries (with a mean of 0.84±0.16 per ovary). In case of abnormal COCs reverse result was found. In terms of ovarian categories regarding with CL or without CL, 76.47±0.01% ovaries showed without CL where 23.53±0.01% showed ovary with CL. In case of ovarian category regarding with CL and without CL, Significance difference were found in the parameter. The highest length (2.86 ±0.10) and weight (4.73±0.12) were found in with CL ovaries compared to without CL ovaries. In case of width (cm), both in with CL and without CL ovaries were same (2.30±0.01). The number of follicles (aspirated) was distinctly higher in ovaries with CL (5.28 ±0.136) compared to ovary without CL (2.29±0.14). In terms of total COCs, ovaries without CL showed highest number of COCs (1.41± 0.11) compared to the ovaries with CL (1.17±0.11) where the highest numbers of normal COCs (0.55± 0.01) and abnormal COCs (0.86 ±0.03) were found in ovary without CL compared to ovary with CL (normal COCs 0.5 ±0.01 and abnormal 0.54±0.03). Finally, it can be concluded that left ovaries contain more follicles and COCs than right ovaries and also contain more number of normal COCs. Whereas ovaries without CL contain higher number of follicles and normal COCs compared to ovaries with CL. So, higher number of normal COCs found in left and without CL ovaries.