Abstract:
The present study was undertaken at
‘‘
Horticultural Biotechnology and Stress
Management Lab
’’
, Sher-e-Bangla Agricultural University, Dhaka.The single factor
experiment was laid out in Complete Randomized Design with three replications. Nodal
segment was used as explant and six types of different low cost medium (LCM) such as
LCM-1 (100 ml coconut water); LCM-2 (150 ml coconut water); LCM-3 (200 ml
coconut water); LCM-4 (100 ml banana pulp) ; LCM-5 (150 ml banana pulp); LCM-6
(200 ml banana pulp) and full strength of MS medium used as a control with three
replication for standardization a low cost in vitro regeneration protocol for Gynura
procumbens. All culture medium was supplemented with 2.0 mg/l BA + 0.5 mg/l NAA
for shooting and 0.5 mg/l IBA was used for rooting. Culture media LCM-2 showed best
performances in shoot initiation, shoot multiplication and root formation compared to
other low cost culture medium. In terms of cost analysis, LCM-2 helps to reduce cost
(78.22%) of the medium per plantlet as compare to the conventional MS medium.
Acclimatization medium consisting garden soil + Vermicompost + cocodust (1:1:1, v/v)
exhibited the best hardening medium with maximum survival rate (96.45%) and vigorous
performances. Taken together, we can conclud that LCM-2 culture medium is a cost
effective protocol and alternative to conventional MS medium for regeneration of Gynura
procumbens aseptically.
Description:
A Thesis Submitted to
The Department of Horticulture, Faculty of Agriculture
Sher-e-Bangla Agricultural University, Dhaka-1207
In partial fulfillment of the requirements
For the degree
of
MASTER OF SCIENCE (MS)
IN
HORTICULTURE
SEMESTER: JANUARY-JUNE, 2018
