Abstract:
The present study was undertaken in Biotechnology Laboratory of the Department of
Biotechnology, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka1207,
during
the
period
from
February
2018
to
June
2018
to
evaluate
the
effect
of
different
plant
growth regulators (BA, IBA) on in vitro plantlet regeneration of Turmeric. The
healthy, disease free sprouts of turmeric were used as explants which were inoculated in
MS media supplemented with different conc. of Benzyl adenine (BA) (1.0, 2.0, 3.0 and 4.0
mg/L) and IBA (1.0, 2.0, 3.0 and 4.0 mg/L) either alone or in combination for shoot and
root regeneration. The experiments were arranged in Randomized Completely Block
Design (RCBD) with three replications. The maximum shoot induction was obtained with
2.0 mg/L BA. The highest shoot length (5.93 cm) was noticed in BA 2.0 mg/L + IBA 3.0
mg/L and minimum shoot length (1.4 cm) with control MS dose was observed. The highest
percentage (81.00%) of shoot induction was recorded with BA 2.0 mg/L in minimum (14.0
days). Where, IBA 3.0 mg/L had produced the highest percentage of root induction
(82.00%) required minimum (20 days). The highest length of shoot (3.4 cm, 5 cm and 5.9
cm) at 21, 28 and 42 DAI respectively was found in BA 2.0 mg/L + IBA 3.0 mg/L. The
treatment BA 2.0 mg/L + IBA 1.0 mg/L gave the highest number of root (6.0, 8.6, 12.67)
at 21, 28 and 42 DAI respectively and the highest length of root (2.7 cm, 4.8 cm and 5.4
cm) at 21, 28 and 42 DAI, respectively was found in BA 2.0 mg/L + IBA 1.0 mg/L.
Regenerated plantlets showed, 100% survival during in growth chamber conditions and
86.66% in shade house stage of hardening and 83% in open atmosphere at direct sunlight.
Therefore, an efficient protocol has been developed for in vitro propagation of Turmeric
which has great commercial value for year round production in Bangladesh.
Description:
A Thesis
Submitted to the Department of Biotechnology, Faculty of Agriculture
Sher-e-Bangla Agricultural University, Dhaka-1207, In partial fulfillment of the requirements for the degree of
MASTER OF SCIENCE (MS)
IN
BIOTECHNOLOGY
SEMESTER: JANUARY- JUNE, 2018