Abstract:
In vitro efficacy six selected chemicals were evaluated against Erwinia
carotovora subsp. carotovora the causal agent of potato soft rot disease. The
experiment was done at Molecular Plant Pathology laboratory of the
department of Plant Pathology of Sher-e- Bangla Agricultural University. E.
carotovora was isolated from infected potato tubers by dilution plate method
and different biochemical tests and pathogenicity test were performed to
confirm the bacterial species. Six chemicals viz. Sunvit 50 WP (Copper
Oxychloride) @ 0.2%, Dithane M-45 (Mancozeb) @ 0.2%, Boric acid (Boric
Powder) @ 0.1%, Kasumin (Kasugamycin) @ 0.02%, Autostin 50 WG
(Carbendazim) @ 0.3% and Chlorox (Sodium Hypochlorite) @ 0.2% were
tested against E. carotovora by well diffusion method. For each treatment there
were four replications and tested chemical volume was 100 μl. Data were
recorded up to five days of incubation. Among six chemicals only four
chemicals viz. Sunvit 50 WP, Dithane M-45, Boric acid and Kasumin showed
bactericidal activity against E. carotovora subsp. carotovora. Maximum zone
of inhibition (mm) was obtained after 48 hours of incubation with Sunvit 50
WP (30.35 mm), followed by Dithane M-45 (20.15 mm), Boric acid (19.15
mm) and Kasumin (16.28mm). While the least zone of inhibition was recorded
with Autostin 50WG (3.83mm) and Chlorox (2.42mm). Sunvit 50 WP
produced the maximum growth inhibition (33.79%) of the pathogen, on the
other hand Cholorx (3.47%) did not effectively inhibit the growth of E.
carotovora. No inhibition was recorded with untreated control/water. Sunvit
50WP proved to be the best chemical followed by Dithane M-45 under in-vitro
screening test against E. carotovora subsp. carotovora.
Description:
A Thesis
Submitted to the Faculty of Agriculture,
Sher-e-Bangla Agricultural University,
Dhaka, in partial fulfillment
of the requirements
for the degree
of
MASTER OF SCIENCE
IN
PLANT PATHOLOGY
SEMESTER: JANUARY-JUNE, 2017
