Abstract:
Rice (Oryza sativa L.) is one of the most important cereal crop around the world. It is the
staple food in Bangladesh. This study was conducted in Biotechnology Laboratory of the
Department of Biotechnology, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar,
Dhaka-1207 from the period of November 2018 to April 2019 to determine the molecular
diversity among four rice hybrid varieties using Random Amplified Polymorphic DNA
(RAPD) and Single Sequence Repeat (SSR) markers. A total of 21 RAPD primers and 4 SSR
primers were used to determine polymorphism among the rice hybrids to produce scorable
DNA bands. Total 82 bands were amplified through RAPD primers. Among them 26 were
polymorphic bands. The range of DNA amplification varied from 1300-150 bp. The rate of
polymorphism was obtained about 30.33%. Lower level of polymorphisms in hybrids
indicated that there is a basic similarity among the varieties which is due to the common
ancestor and the similarity of the selective traits. Genetic diversity ranged from 0 to 0.5000
and the frequency of major allele ranged from 0.5000 to 1.0000. Nei’s genetic distance
ranged from 0.1190 to 0.2262. The PIC value ranged from 0 to 0.3750 with the average value
0.1052. The PIC value indicates that the studied rice hybrids had low molecular diversity. A
dendogram indicating the relative genetic similarity of the rice hybrids was constructed which
followed three major clusters (A, B and C) among the studied material. Two SSR primers
showed polymorphic band which can be used as an evidence of variety protection data. This
experimental findings can be used for the protection of hybrids in commercial purpose and in
managing rice genetic resource in Bangladesh.
Description:
A Thesis Submitted to
The Department of Biotechnology, Faculty of Agriculture
Sher-e-Bangla Agricultural University, Dhaka-1207
In partial fulfillment of the requirements
For the degree of
MASTER OF SCIENCE (MS)
IN
BIOTECHNOLOGY
SEMESTER: JANUARY-JUNE, 2018
