Abstract:
The present study was undertaken in Biotechnology Laboratory of the
Department of Biotechnology, Sher-e-Bangla Agricultural University, Sher-eBangla
Nagar, Dhaka-1207, during the period from June, 2017 to January,
2018 to evaluate the effect of different plant growth regulators (BA, IBA) on in
vitro plantlet regeneration in Mint. The shoot tips of Mint were used as
explants which were inoculated in MS media supplemented with different
conc. (1.0, 1.5, 2.0 and 2.5 mg/l) of Benzyl adenine (BA) and Indole-3-butyric
acid (IBA) (0.5, 1.0, 1.5 and 2.0 mg/l) either alone or in combination of both.
The experiments were arranged in Completely Randomized Design (CRD)
with three replications. The maximum shoot induction was obtained with 2.0
mg/L BA. The highest shoot length (12.12 cm) was found in 2.0 mg/L BA and
minimum shoot length (2.90 cm) in control treatment. The highest percentage
(85.00%) of root induction was recorded with BA 2.0 mg/l + IBA 1.0 mg/l in
minimum (11.0 days). The highest length of root (3.25, 5.00 and 7.50 cm) at
21, 28 and 42 DAI respectively was found in 1.0 mg/l IBA and the control
treatment found the lowest length of root at all DAI. The treatment BA 2.0 mg/l
+ IBA 1.0 mg/l gave the highest number of root (10, 15 and 20.67) at 21, 28
and 42 DAI respectively. Regenerated plantlets showed 90% survival during in
growth chamber and 83.33% in shade house and 80 % in open atmosphere at
direct sunlight. Therefore, an efficient protocol has been developed for in vitro
regeneration of Mint which has great commercial value for year round
production in Bangladesh.
Description:
A Thesis
Submitted to the Department of Biotechnology, Faculty of Agriculture
Sher-e-Bangla Agricultural University, Dhaka-1207,
In partial fulfillment of the requirements
for the degree of
MASTER OF SCIENCE (MS)
IN
BIOTECHNOLOGY
SEMESTER: JANUARY- JUNE, 2018
