Abstract:
An experiment was carried out to study of the cross compatibility between ridge gourd and
sponge gourd and assess hybridity in F
generation through SSR marker. Field experiment was
conducted at the farm of Sher-e-Bangla Agricultural University and laboratory experiment was
conducted at Department of Biotechnology, Sher-e-Bangla Agricultural University, Dhaka1207,
Bangladesh during the period of March 2017 to June 2018. Seven ridge gourd and three
sponge gourd genotypes were used as parents. Most of the crosses were compatible such as
Bogra×BARI Jhinga-1, Bogra×RIG 47-2, Faridpur×RIG 19-2 etc. and some crosses are
incompatible such as Lalmonirhat×BARI Jhinga-1, Lalmonirhat×RIG 19-2 etc. Cross
compatibility was maximum 75% in Bogra×BARI Jhinga-1. Fruit size and weight of the
compatible crosses were intermediate between ridge gourd and sponge gourd. The interspecific
hybrids produced fruits that have less number of seeds with less fiber density than that of the L.
cylindrica entries. For hybridity test, initially different SSR primers were used to establish
polymorphism among the parents of each hybrid. The identified polymorphic primers were used
to screen the hybridity test. It was noticed that three primers viz. BoG-21, BoG-76 and BoG-117
showed polymorphism in the parents and their corresponding hybrids. It was noted that, a 550bp
DNA fragment was amplified in male parent and hybrid of Bogra×RIG 47-2 which was absent
in female genotype. Again, 200 bp DNA fragment was amplified in male parent and
Bogra×BARI Jhinga-1 hybrid. This DNA band can be used for large scale screening of that
specific hybrid variety for seed purity in commercial purpose. The parents of one hybrid
(Faridpur×KRG 02) showed monomorphic DNA band and one of the SSR primers viz. BoG 118
was not amplified in any ridge gourd and sponge gourd genotypes.
1
Description:
A Thesis Submitted to
The Department of Biotechnology, Faculty of Agriculture
Sher-e-Bangla Agricultural University, Dhaka-1207
in partial fulfillment of the requirements
for the degree
of
MASTER OF SCIENCE (MS)
IN
BIOTECHNOLOGY
SEMESTER: JANUARY-JUNE, 2018