Abstract:
The present study was undertaken in Biotechnology Laboratory of the Department of
Biotechnology, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka1207
during
the
period
from
February,
2017
to
March,
2018
to
evaluate
the effect of
different plant growth regulators (BAP and IBA) on in vitro plantlet regeneration of
pepper. The shoot tips of pepper were used as explants which were inoculated in MS
media supplemented with different concentration of BAP (1.0, 2.0, 3.0 and 4.0 mg/L)
and IBA (1.0, 1.5, 2.0 and 2.5 mg/L) alone or in combination for plantlet
regeneration. The experiments were arranged in CRD with three replications.
The
highest frequency (72%) of shoot development was observed in MS medium
augmented with BAP 3.0 mg/L at minimum (18.25) days. Maximum number of
shoots 3.50 mg/L was found in 35 days after shoot induction (DAI) at 3.0 mg/L BAP
where the control treatment gave the lowest result. In combined effect, BAP 3.0 mg/L
+ IBA 2.0 mg/L showed the highest percentage (78.00%) of callus induction at in
16.00 days and the maximum size of callus (3.26 cm) was noticed at 35 days after
induction. The highest number of shoot (3.76) was noticed from the same treatment at
35 DAI. The highest number of root (20.32) at 35 DAI was obtained in 2.0 mg/L IBA.
The maximum length of roots (4.25 cm) was observed in 1.5 mg/L IBA
Finally, the survival rate of in vitro regenerated plantlets was recorded 75% in natural
condition. Feasible protocol has been developed for in vitro rapid regeneration of
pepper having potential in cell culture technology.
Description:
A Thesis
Submitted to the Department of Biotechnology, Faculty of Agriculture
Sher-e-Bangla Agricultural University, Dhaka-1207,
In partial fulfillment of the requirements
for the degree of
MASTER OF SCIENCE (MS)
IN
BIOTECHNOLOGY
SEMESTER: JANUARY- JUNE, 2018
