Abstract:
An experiment was carried out with twenty four Boro rice germplasm at the laboratory of
Genetic Resources and Seed Division (GRSD), Bangladesh Rice Research Institute (BRRI),
Joydebpur, Gazipur, during the period of November 2017 to March 2018 to investigate
molecular characterization and genetic diversity analysis. Rice genome specific twelve well
known SSR primers were selected and synthesized for molecular diversity analysis. All of the
twelve primers were able to produce polymorphic bands. The highest (8) number of bands was
observed in SSR primers RM206. Fifty four alleles, ranged from 1 to 8 alleles per locus and an
average of 4.5 were detected. Forty five polymorphic alleles were also identified. The
Polymorphic Information Content (PIC) of SSR markers ranged from 0.08 (RM447) to 0.84
(RM206) with an average value of PIC = 0.49. Gene diversity ranges from 0.08 (RM447) to 0.86
(RM206) with an average value of 0.52. The RM206 marker can be considered as the best
marker among the studied markers for 24 rice germplasms. Six primers showed high diversity,
three primers showed moderate diversity and three primers showed low diversity on the basis of
PIC value. The value of pair-wise comparisons of Nei’s genetic distance (D) among 24 rice
genotypes was computed from combined data for the 12 primers, ranged from 0.1667 to 0.9167
with an average of 0.5417. Dendrogram based on Nei’s genetic distance using Unweighted Pair
Group Method of Arithmetic Mean (UPGMA) indicated the segregation of 24 genotypes into
three main clusters. This result can be conveniently used for molecular diversity analysis of rice
genotypes to identify diverse parent for the development of high yielding variety in rice.
Description:
A Thesis Submitted to
The Department of Biotechnology, Faculty of Agriculture
Sher-e-Bangla Agricultural University, Dhaka-1207
in partial fulfillment of the requirements
for the degree of
MASTER OF SCIENCE (MS)
IN
BIOTECHNOLOGY
SEMESTER: JANUARY – JUNE, 2018
