IN VITRO REGENERATION OF PAPAYA (Carica papaya)

Abstract

The present research was carried out in Biotechnology Laboratory of the Department of Biotechnology, Sher-e-Bangla Agricultural University, Sher-eBangla

Nagar, Dhaka-1207 from the period of September 2017 to June 2018 to study on in vitro regeneration of Papaya (Carica papaya). Shoot tip of young shoot was used as the main suitable explant, which was sterilized using freshly prepared 0.1% HgCl mixing with few drops Tween-20. Explants were inoculated in MS media treated with both singly or in combination of the Benzyladenine 2 (BA), Kinetin (KIN), Indole-3-butyric acid (IBA), Naphthalene acetic acid (NAA). The minimum days to shoot induction were achieved on MS medium containing 0.5 mg L -1 BA. The highest shoots, leaves, length of shoot observed in treatment 1.0 mg L -1 BA. The combine treatment 1.0 mg L -1 BAP+0.75 mg L

KIN gave the highest number of shoots and number of leaf plantlet. The minimum days to root induction was reported in media treated with 2.0 mg L IBA along with maximum roots per plantlet. The highest length of root was observed in 2.0 mg L -1 IBA. The minimum days to root induction were noticed on same culture media supplemented with 2.00 mg L -1 NAA. In regenerated plantlets, 80% survival during growth chamber conditions and 75% during shade house stage and 75% in open atmosphere were achieved. In vitro regeneration was found to be very effective and promising method in the proliferation of papaya and this experiment can be a useful tool for proliferation of papaya. -1 -1