Abstract:
The present research was carried out in Biotechnology Laboratory of the
Department of Biotechnology, Sher-e-Bangla Agricultural University, Sher-eBangla
Nagar, Dhaka-1207 from the period of September 2017 to June 2018 to
study on in vitro regeneration of Papaya (Carica papaya). Shoot tip of young
shoot was used as the main suitable explant, which was sterilized using freshly
prepared 0.1% HgCl
mixing with few drops Tween-20. Explants were inoculated
in MS media treated with both singly or in combination of the Benzyladenine
2
(BA), Kinetin (KIN), Indole-3-butyric acid (IBA), Naphthalene acetic acid
(NAA). The minimum days to shoot induction were achieved on MS medium
containing 0.5 mg L
-1
BA. The highest shoots, leaves, length of shoot observed in
treatment 1.0 mg L
-1
BA. The combine treatment 1.0 mg L
-1
BAP+0.75 mg L
KIN gave the highest number of shoots and number of leaf plantlet. The minimum
days to root induction was reported in media treated with 2.0 mg L
IBA along
with maximum roots per plantlet. The highest length of root was observed in 2.0
mg L
-1
IBA. The minimum days to root induction were noticed on same culture
media supplemented with 2.00 mg L
-1
NAA. In regenerated plantlets, 80%
survival during growth chamber conditions and 75% during shade house stage and
75% in open atmosphere were achieved. In vitro regeneration was found to be
very effective and promising method in the proliferation of papaya and this
experiment can be a useful tool for proliferation of papaya.
-1
-1
Description:
A Thesis
Submitted to the Faculty of Agriculture,
Sher-e-Bangla Agricultural University, Dhaka,
in partial fulfilment of the requirements
for the degree of
MASTER OF SCIENCE
IN
BIOTECHNOLOGY
SEMESTER: JANUARY-JUNE, 2018