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MOLECULAR DIVERSITY ANALYSIS AND DNA FINGERPRINTING OF DIFFERENT TURMERIC (Curcuma longa L.) GENOTYPES USING RAPD MARKERS

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dc.contributor.author SUMI, SHAMIM ARA
dc.date.accessioned 2019-10-30T11:39:43Z
dc.date.available 2019-10-30T11:39:43Z
dc.date.issued 2018-06
dc.identifier.uri http://archive.saulibrary.edu.bd:8080/xmlui/handle/123456789/2908
dc.description A Thesis Submitted to the Faculty of Agriculture, Sher-e-Bangla Agricultural University, Dhaka, in partial fulfillment of the requirements for the degree of MASTER OF SCIENCE IN BIOTECHNOLOGY SEMESTER: JANUARY – JUNE, 2018 en_US
dc.description.abstract Turmeric (Curcuma longa) has been widely used as a spice and flavouring agent in food. This study was done to analyze the molecular diversity among 8 turmeric genotypes using Random Amplified Polymorphic (RAPD) DNA primers. A total 12 RAPD primers were used to determine polymorphism among the turmeric genotypes to produce scorable DNA bands. Some total of 34 DNA bands were amplified and among them 23 were polymorphic bands. The range of DNA amplification varied from 100 to 900 bp. The rate of polymorphism was obtained about 63.88%. Genetic diversity ranged from 0.531 to 0.750 and the frequency of major allele ranged from 0.375 to 0.625. Nei’s genetic distance ranging from 0.0930 to 0.2013. The PIC value ranged from 0.468 to 0.712 with the average value was 0.589. The PIC value indicates that most of the studied turmeric genotypes was highly diversified and homogenous as well as no heterozygosity found. A dendogram indicating the relative genetic similarity of the turmeric genotypes was constructed which followed two major clusters (A and B) among the studied material. The cluster B was further subdivided. The results also showed that the genotypes can be separated from each other at the molecular level by taking advantage of some of the RAPD markers. The prevalence level of polymorphism in the local genotypes of turmeric will help breeders for the improvement of turmeric breeding program. This study can be used as a baseline for the future molecular research work on turmeric genotypes in Bangladesh. en_US
dc.language.iso en en_US
dc.publisher DEPARTMENT OF BIOTECHNOLOGY, SHER-E-BANGLA AGRICULTURAL UNIVERSITY, DHAKA-1207 en_US
dc.subject MOLECULAR DIVERSITY ANALYSIS en_US
dc.subject DNA FINGERPRINTING en_US
dc.subject TURMERIC en_US
dc.subject Curcuma longa L. en_US
dc.subject RAPD MARKERS en_US
dc.title MOLECULAR DIVERSITY ANALYSIS AND DNA FINGERPRINTING OF DIFFERENT TURMERIC (Curcuma longa L.) GENOTYPES USING RAPD MARKERS en_US
dc.type Thesis en_US


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