Abstract:
Turmeric (Curcuma longa) has been widely used as a spice and flavouring agent in food.
This study was done to analyze the molecular diversity among 8 turmeric genotypes using
Random Amplified Polymorphic (RAPD) DNA primers. A total 12 RAPD primers were
used to determine polymorphism among the turmeric genotypes to produce scorable DNA
bands. Some total of 34 DNA bands were amplified and among them 23 were polymorphic
bands. The range of DNA amplification varied from 100 to 900 bp. The rate of
polymorphism was obtained about 63.88%. Genetic diversity ranged from 0.531 to 0.750
and the frequency of major allele ranged from 0.375 to 0.625. Nei’s genetic distance
ranging from 0.0930 to 0.2013. The PIC value ranged from 0.468 to 0.712 with the average
value was 0.589. The PIC value indicates that most of the studied turmeric genotypes was
highly diversified and homogenous as well as no heterozygosity found. A dendogram
indicating the relative genetic similarity of the turmeric genotypes was constructed which
followed two major clusters (A and B) among the studied material. The cluster B was
further subdivided. The results also showed that the genotypes can be separated from each
other at the molecular level by taking advantage of some of the RAPD markers. The
prevalence level of polymorphism in the local genotypes of turmeric will help breeders for
the improvement of turmeric breeding program. This study can be used as a baseline for
the future molecular research work on turmeric genotypes in Bangladesh.
Description:
A Thesis
Submitted to the Faculty of Agriculture,
Sher-e-Bangla Agricultural University, Dhaka,
in partial fulfillment of the requirements
for the degree of
MASTER OF SCIENCE
IN
BIOTECHNOLOGY
SEMESTER: JANUARY – JUNE, 2018
