Abstract:
The present research was carried out in Biotechnology Laboratory of the Department of
Biotechnology, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka1207
from the period of september 2017 to June 2018 for in vitro renegaration of
tobacco. The healthy, disease free shoot tips of 0.25-0.50 cm length were used as
explants, It was sterilized by 0.1% HgCl
mixing with few drops Tween-20. The explant
was inoculated in MS media supplemented with different combination of the BA and
IBA. The minimum days to shoot induction were achieved on BA 3.00 mg/L. The
highest shoots and leaves per explant also observed in BA 3.00 mg/L. The combine effect
of auxin and cytokinine hormone was also investigated. It was noticed that the minimum
days to shoot induction were recorded in 3.00 mg/L BA+1.00 mg/L IBA. The highest
number of shoot, number of leaf per explants and length of shoot and length of leaf were
recorded in 3.00 mg/L BA+ 1.00 mg/L IBA. The highest number of root and length of
root was observed in 3.00 mg/L BA+ 1.00 mg/L IBA. Micropropagation was found to be
very effective and promising method in the proliferation of tobacco and this experiment
can be a useful tool for tissue culture of tobacco
2
Description:
A Thesis
Submitted to the Faculty of Agriculture,
Sher-e-Bangla Agricultural University, Dhaka,
in partial fulfilment of the requirements
for the degree of
MASTER OF SCIENCE
IN
BIOTCHNOLOGY
SEMESTER: JANUARY-JUNE, 2018
