Abstract:
ZINNAT KARIM
Infected samples from different sources of inoculum viz. plant, soil, seed, weed, other crops, irrigation water etc.
were collected from major potato growing districts of Bangladesh viz. Munshiganj, Chandpur, Tangail,
Narayanganj, Jamalpur, Domar, Patuakhali, Rangpur, Bogra, Shariatpur, Meherpur, Joypurhat and Dinajpur to
investigate the presence of R. solanacearum. Cross cut of plant samples showed bacterial ooze streaming in clear
water and browning of the vascular bundle region of seed tuber were observed. Standard methods viz. Kelman’s
tetrazolium chloride
0
C and 28
0
C temperature. Again, under the influence of
temperature avirulent colony of R. solanacearum showed VBNC state at 10
0
C. In vitro and in
vivo performance test with ten bioactive compounds against R. solanacearum were conducted as compared to
control, bactericide and farmers practice to design an effective management tool. Propolis
C and over 40±2
0
Key words: Bacterial wilt disease, Bangladesh, heterogeneity in virulence and biovar(s
Description:
A Thesis
Submitted to the Faculty of Agriculture,
Sher-E-Bangla Agricultural University, Dhaka
in partial fulfilment of the requirements
for the degree of
DOCTOR OF PHILOSOPHY
IN
PLANT PATHOLOGY
SEMESTER: JANUARY-JUNE, 2017
SHER-E-BANGLA AGRICULTURAL UNIVERSITY
DHAKA-1207
