Abstract:
The present study was carried out to isolate and identify the pathogenic bacteria from
table egg with special emphasis to E. coli, Salmonella spp. and Staphylococcus spp.
Techniques forward to isolate, identify and characterize the bacterial species by cultural,
morphological, biochemical and staining. The antimicrobial susceptibility pattern of
isolates were investigated by disc diffusion method. A total number of 40 eggs were
collected from different markets of Dhaka, Bangladesh such as Krishi market, Bihari
camp market, Agargaon market and SAU market. In Gram’s staining, the organism
revealed gram-negative, pink color, small rod shaped appearance, arranged in single or
paired short called E.coli, the organism revealed gram negative, short rod shaped, singly
arranged called Salmonella spp., the organisms were found as gram positive grapes like
clusters formed by cocci. E. coli from 14 samples, Salmonella spp. from 11 samples and
Staphylococcus spp. from 7 samples were isolated from total of 40 samples that means
(35.00%) were positive for E. coli ,(27.50 %) were positive for Salmonella spp. and
(17.50%) were positive for Staphylococcus spp. The antibiotic sensitivity test indicated
that the isolated E. coli were highly resistant to penicillin, erythromycin and amoxicillin;
and were sensitive to tetracycline, ciprofloxacin and gentamycin. The isolated Salmonella
spp. were highly resistant to penicillin, erythromycin and amoxicillin; and were sensitive
to tetracycline, ciprofloxacin and gentamycin. Staphylococcus spp. were resistant against
Neomycin, Vancomycin and were sensitive to ciprofloxacin, erythromycin, tetracycline
and gentamycin.
