Abstract:
The experiment was conducted at the Laboratory of the Department of Biotechnology, Sher-eBangla
Agricultural
University
(SAU),
Dhaka-
1207,
Bangladesh.
The
period
of
the
experiment
was
March 2018 to November 2018. Cotton (Gossypium hirsutum L.) is the leading fiber crop
and the second most important oil seed crop in the world. This study aimed to analyze the
molecular diversity among 9 cotton genotypes collected from Cotton Development Board,
Bangladesh using the Random Amplified Polymorphic DNA primer. A total of 11 RAPD
primers were used to determine polymorphism among the genotypes and finally 6 of the primers
produced scorable DNA bands. Some total of with 30 DNA bands were obtained and among
them 9 were polymorphic bands. The ranged of DNA amplification varied from 160 to 1500 bp.
The rate of polymorphism was calculated about 31.57%. Genetic diversity ranged from 0.22 to
0.44 with an average value of 0.30. Nei’s genetic distance ranging from 0.1667 to 0.6667 and
most importantly PIC value ranged from 0.18 to 0.35 with an average value of 0.27. The PIC
value indicates that most of the studied cotton genotypes was moderately diversified and
homogenous as well as no heterozygosity found. A dendogram indicating the relative genetic
similarity of the Bangladeshi cotton genotypes was constructed which followed in two major
cluster (A and B) among the studied material. The results also showed that the genotypes can be
separated from each other at the molecular level by taking advantage of some of the RAPD
markers. The Cotton Development Board, Bangladesh committed to work on cotton
improvement and so for this was the first time in CDB to investigate molecular diversity and
DNA profiling in cotton using CDB collected cotton genotypes. This experiment can be used as
a baseline for the future molecular research work on cotton genotypes in Bangladesh.
