Abstract:
Off-season sprouting ability and in vitro regeneration potentiality were investigated to establish a rapid
multiplication protocol in three potato varieties viz, Diamant, Cardinal and Granola. The results revealed
that sprout initiation time, sprout length and sprout number were directly related to the concentration of
GA, applied. Higher concentration (400 ppm) of GA, showed better performance for all the parameters
studied. Among the three varieties, Granola showed quick response to GA, application. MS medium
supplemented with I, 2, 3, 4 and 5 mg/L of GA, were employed for in vitro regeneration of these three
cultivars. Among all the treatments, MS medium + 4.0 mg/L of GA, showed the best performance on
multiple shooting. The hormone IBA was applied for root induction on the regenerated shoot of the potato
varieties. In all the varieties 2 rng/L of IBA showed the highest root induction. Variety Granola was the
most responsive in both shoots and root development. The regenerated plantlets were acclimatized in
natural environment Among them almost 90% plants ultimately survived.
