IDENTIFICATION OF SELF-INCOMPATIBLE LINE FROM AVAILABLE GERMPLASMS OF Brassica napus

Abstract

Self-incompatibility mechanism in 22 local germplasm of Brassica napus genotypes was investigated through seed set analysis and pollen tube growth behavior. Based on the seed set ratio. levels of self- incompatibility varied from 0.0 to 93.33 percent. The entries Nap 205, Nap 248 and Nap 2037 showed low level of self-compatibility. The genotypes Nap 0130, Nap 2013, Nap 9901. Nap 9904 and Nap 94006 showed intermediate level of self-incompatibility whereas the genotypes Nap 2001 and BARI Sarisha-l J were almost self-incompatible. Based on the number of pollen tubes in the style, the genotypes Nap 108. Nap 179. Nap 206, Nap 2012, Nap 2022, Nap 2057. Nap 2066, Nap 9905 and Nap 9908 were grouped as self-compatible: Nap 0130. Nap 2013. Nap 9901, Nap 9904. Nap 94006, BAR I Sharisha-? and BARI Sharisha-S as intermediate. Rest of the genotypes Nap 205. Nap 248, Nap 2001, Nap 2037 and BARI Sharisha-l J were classed as self-incompatible. Both the methods provide more or less similar results. Five genotypes. namely Nap 205. Nap 248. Nap 2001, Nap 2037 and BARI Sharisha-13 showed high level of self-incompatibility along with success on bud pollination for producing Sllines.