Abstract:
High frequency regeneration of plants from in vitro cultured tissues is perqursite to use genetic
transformation to enrich oleiferous Brassica spp. Emphasis was given in this study on callus induction
aptitude and subsequent plant regeneration from cotyledon and stem segment of three of Brassica spp. viz
BARI sarisa-8 (Brassica napus), Daulat (Brassica juncea) and Sonali iBrassica campestriss. Two
milligram per litre of Kn, BAP (1.0, 2.0, 3.0 mg/I) and constant concentration of NAA (0.5 mg/l) were
used in MS medium. The result showed that stem segment produced maximum percentage of callus and
subsequent shoot regeneration in all the four treatments BARI sarisa -8 Showed best performance in
callus induction and it take minimum (6-7 days) for callus initiation. Shoot initiation potentiality also
highest in the same variety under studied. The variety sonali showed poor performance for all the
parameter under studied. Rooting occurred simultaneously from regenerated shoot on half strength MS
medium supplemented with 0.5 mgll iliA. Regenerated plantlets were successfully transferred to pots
containing a mixer of soil and vermiculite.
