Abstract:
The experiment was conducted at the Biotechnology Laboratory, Department of Biotechnology,
Sher-e-Bangla Agricultural University, Dhaka, Bangladesh during the period of January, 2016 to
June, 2017 to evaluate the performance of ammonium nitrate free medium for in vitro
regeneration of a potato (Solanum tuberosum L.) variety, Asterix. Sprouts and nodal segments
were used as explants. A new chemical which is indicated as β chemical had been used in this
research. Five different compositions of the stock solution-01 of the MS medium were used
which were marked as Treatment-01 (standard MS dose, 1962), Treatment-02 (NH
4
free
stock solution), Treatment-03 (NH
4
NO
3
free stock solution with double dose of other
ingredients), Treatment-04 (stock solution-01, developed in the Department of Biotechnology,
SAU, Dhaka ) and Treatment-05 (readymade MS powder). In vitro regeneration of a potato was
successfully achieved in those media. The parameters like shoot length, shoot number, shoot
diameter, node number per plantlet, leaf number per plantlet were highest in Treatment-04 at
14, 21 and 28 days of regeneration. But in case of root number and root length (cm) per
plantlet, Treatment-05 showed the highest result and in some cases it was statistically similar
with Treatment-04. Treatment-02 showed lowest result in each of the said parameter. It might be
due to the low concentration of nitrogen and it indicated that NH
4
x
NO
3
is essential for in vitro
regeneration of potato. Treatment-03 performed moderately in most of the parameters. Asterix
variety showed significant variation in case of Treatment-01
and Treatment-05
on most of the
parameters under studied. Therefore, a relliable medium composition for in vitro potato
regeneration has been established. So, the stock solution-01 which was developed from the
Department of Biotechnology, SAU, Dhaka can be the best alternative to NH
4
in tissue
culture study of plants.
Description:
A Thesis
Submitted to the Faculty of Agriculture,
Sher-e-Bangla Agricultural University, Dhaka,
in partial fulfillment of the requirements
for the degree of
MASTER OF SCIENCE (MS)
IN
BIOTECHNOLOGY
