Abstract:
The present research was carried out in Biotechnology Laboratory of the Department of
Biotechnology, Sher-e-Bangla Agricultural University, Dhaka-1207 from the period of
February 2016 to June 2017 for in vitro regeneration of chrysanthemum where nodal
segments were used as explants. Five levels of BA (1.0, 2.0, 3.0, 4.0 and 5.0 mg/l) and
IAA (0.5, 1.0, 1.5, 2.0 and 2.5mg/l) were used for shoot and root induction. 2,4-D (0.5,
1.0, 1.5 and 2.0 mg/l) was used in combination with 2.0 mg/l of BA for callus and shoot
induction. The highest frequency of shoot (80.00%) in minimum 7.60 days and
maximum number of shoot (2.20, 2.60 and 3.00) at 14, 21 and 28 DAI (days after
induction) were recorded from BA 2.0 mg/l. Moreover, the highest length of shoot and
number of leaves was noticed from the 3.0 mg/l and 2.0 mg/l of BA, respectively at 14,
21 and 28 DAI. The highest frequency of callus (76.00%) was observed within 14.20
days in MS medium and weight of callus (1.80 g, 2.70 g and 3.50 g) was obtained with
BA 2.0 mg/l+ 2,4-D 1.0 mg/l. The maximum number of shoot (3.20) at 40 DAI was
obtained with BA 2.0 mg/l+ 2,4-D 1.0 mg/l within minimum 9.20 days. IAA 0.5 mg/l
produced the highest percentage of root (80.00%) within 13.00 days while longer days
required in other treatments. The maximum number of root (2.60, 3.60 and 5.40) was
obtained from 1.5 mg/l IAA at 14, 21 and 28 DAI, respectively. The maximum length of
shoot (2.48 cm, 2.84 cm and 3.66 cm) at 14, 21 and 28 DAI was noticed from the BA 2.0
mg/l+ IAA 1.0 mg/l. In combined effect the highest percentage (76%) of root induction
was recorded with BA 3.0 mg/l+ IAA 1.5 mg/l in minimum 12.20 days and BA 2.0
mg/l+ IAA 1.0 mg/l gave the highest number of root 2.40, 3.20 and 4.20 at 14, 21 and 28
DAI, respectively. The survival rate was 80% in shade condition which was 75% in open
atmospheric condition. Therefore, an efficient protocol has been developed for in vitro
regeneration of chrysanthemum which has great commercial value for year round
production in Bangladesh.
Description:
A Thesis
Submitted to the Department of Biotechnology,
Sher-e-Bangla Agricultural University, Dhaka
In partial fulfillment of the requirements
for the degree
of
MASTER OF SCIENCE (MS)
IN
BIOTECHNOLOGY
