Abstract:
Ginger (Zingiber officinale) rhizomes have been widely used as a spice and flavoring agent in foods
and beverages in Bangladesh as well as in all over the world for its economical and medicinal
values. Identification and characterization of germplasm is an important link between the
conservation and utilization of plant genetic resources. The present investigation was undertaken
for the assessment of 13 local genotypes of Zingiber officinale collected from different region of
Bangladesh by 7 RAPD random decamer primers. Good quality genomic DNA was extracted from
ginger genotypes using CTAB method. Nine RAPD primers were screened for amplification of
genomic DNA and seven primers were selected based on the amplification pattern. A total of 34
distinct and differential amplification bands ranging from 150-1200 kb were observed with an
average of 1.14 polymorphic bands per primer. The overall gene diversity was detected 0.8052 and
the value of PIC was detected 0.7532. Primers could generate enough polymorphism for possible
use in diversity studies, based on provisional multivariate analyses such as cluster analysis and
principal component analysis (PCA). PCA classified 13 ginger genotypes into four groups and
showed in two dimensional scatter plot. The genetic similarity coefficients among genotypes
ranged from 0.103 to 0.654. Cluster analysis based on Jaccard’s similarity-coefficient using
UPGMA grouped the genotypes into two clusters: Cluster A and Cluster B. The cluster ‘A’ had
only one genotype Kaptai local and the second cluster ‘B’ had rest of twelve genotypes. Further
cluster B subdivided into another cluster. The prevalence level of polymorphism in the local
genotypes of ginger will help to breeders for ginger development breeding program.
Description:
A Thesis
Submitted to the Faculty of Agriculture,
Sher-e-Bangla Agricultural University, Dhaka,
in partial fulfillment of the requirements
for the degree of
MASTER OF SCIENCE
IN
BIOTECHNOLOGY
