Isolation, Characterization and Antibiogram Study of Pasteurella multocida Isolated from Ducks of Kishoreganj District, Bangladesh

Abstract

A study was carried out to isolate and characterize Past eurel l a mul t oci da isolates from ducks with emphasis on antibiotic sensitivity and resistant pattern. A total of 100 fecal samples were aseptically collected from sick ducks during suspected duck cholera outbreak in Kishoreganj district, Bangladesh during the period from January to December, 2009. The processed samples were cultured on blood agar media for isolation of bacteria. Pure cultures were obtained after subsequent cultures. P. mul t oci da isolates were identified according to their morphology, staining, motility, cultural and biochemical properties. The isolated bacteria were then subjected to antibiotic sensitivity test. The prevalence of isolated P. multocida from ducks was 34%. From cultural studies it was found that P. multocida showed small, glistering, mucoid and dew drop like colony on blood agar plate. They did not grow on MacConkey's agar and found to be non-haemolytic on blood agar plates. The isolates were found Gram negative with coccobacillary appearance, bipolar organisms and non-motile. All the isolates were found positive to catalase, oxidase, indole and nitrate reduction tests and negative to methyl red, Voges-Proskaur, citrate utilization and H S production tests. The isolates fermented dextrose, sucrose and manitol without production of gas but did not ferment lactose and maltose. Isolated P. multocida isolates were sensitive to ci pr of l oxaci n ( 95%) and azithromycin (80%), intermediate sensitive to gentamycin (85%), tetracycline (75%), amoxicillin (75%) and erythromycin (70%), but resistant to penicillin G (100%). 2