Comparative study of antihyperglycemic and antihypercholesteromic effect of aquous extract of Allium sativum (Garlic) and Zingiber officinale (Ginger) in alloxan induced rats

Abstract

The aim of this study was to compare the single and combined effect of Allium sativum (Garlic) and Zingiber officinale (Ginger) against hyperglycemia and hypercholsteremia in alloxan induced rats. A total of 300 rats were used for 5 trials. Among them 60 rats (12 normal rats and 48 alloxan induced diabetic rats) were used for each trail. In each trial rats were divided into 5 groups each containing 12 individuals as follows: Group A (Normal Control); Group B (Diabetic control); Group C (Alloxan + Garlic treated); Group D (Alloxan + Ginger treated) and Group E (Alloxan + Garlic + Ginger treated). After 18 hours of starvation, body weights, blood glucose level and blood cholesterol level were measured. Then alloxan were injected @ 100 mg/kg body weight intraperitonially to induce hyperglycemia as well as hypercholsteremia in groups B, C, D, and E. All groups were reared under normal condition from Day 1-10. To ensure the induction of hyperglycemia as well as hypercholsteremia on the day of 10 body weights, blood glucose and cholesterol level were measured. After that all groups were kept for more 21 days. During that period on Day 0, 7, 14, and 21 body weights, blood glucose as well as cholesterol level were measured by using commercially available biochemical kits. Aqueous extract of garlic and ginger were fed @ 500mg and 300mg/kg body weight daily for 21 days in groups C and D respectively and a combined dose (800mg/kg) were used for group E. The blood sugar level and blood cholesterol level were reduced in Group C from 154.5±3.98 to 120.35±4.21 and 209.11±5.24 to 126.66±6.58, respectively. In Group D the blood sugar level and blood cholesterol level were reduced from 155.5±2.99 to 123.12±4.23 and 213.43±6.21 to 128.76±6.98, respectively. But the reduction of blood sugar level and blood cholesterol level were more prominent in Group E from 156±4.11 to 110.21±3.98 and 218.12±6.34 to113.81±6.13, respectively. From the above observations it can be concluded that combined use of garlic and ginger is more effective to control hyperglycemia as well as hypercholsteremia compare to single dose.