Abstract:
An experiment was accomplished at Advanced Seed Research and Biotech Center,
ACI Dhaka, Bangladesh during the period from September, 2014 to September,
2015 to find out suitable explant for culture establishment to develop a standard
protocol. Five explants (E
1
, Flower bud; E
II
2
, Capitulum; E
3
, Leaves; E
, Peduncle;
and E
, Petiole) and six different growth regulators combination were used for
culture establishment. The flower bud is the best explants, due to higher survival
rate (75.6%), early for culture establishment (16.4 days) and lowest contamination
rate (7.1%). MS medium supplemented with 3 mg/l BAP + 0.01 mg/l NAA;
(85.8%) is best medium showed longest shoot (25.4 mm) and higher response for
shoot regeneration in shortest time. The lowest performance found by the control
treatment. Various levels of auxin were tried for root initiation. MS medium of
half strength supplemented with 0.3 mg /l NAA; rooted early (12.0 days) was
found to be the best medium for rooting of in vitro shoots because it showed
highest root number (9.30). Media consists of sand, coco dust, vermicompost
(1:1:1, v/v) resulting as the best hardening materials with maximum survival rate
with better plant vigour. The overall micropropagation technique is effective and
promising for regeneration of gerbera
