Abstract:
Genetic divergence analysis through Principal Component. Principal Coordinate and
Canonical Vector analyses were done for tungro resistant and susceptible genotypes
using 13 morphological characters. The genotypes were grouped into six clusters
according to D2 statistic and Canonical Vector analysis Plant height, days to
flowering, days to maturity, panicle length. number of spikelet per panicle number
of unfilled grain per panicle and yield were mdicated as important contributiors to
genetic divergence in 14 rice genotypes. On the basis of cluster distances, cluster l
(BR10, BRRI dhan44) with cluster ill (Sonahidernota, Kumragoir, Nakuchimota)
and cluster VI (Bazail Ace 252, bazail Accl7l, Rayeda 4849) or cluster l1 (BRI I,
BRRI dhan3 l , BRRI dban32) with cluster Ill were identified for utilization in
hybridization programme. A total of six primers including three SSRs and three
VNTRs were used to characterize tungro resistant genotypes. PIC values of SSR
primers, RM20, RM21 and RM23 ranged from 0 65-0 84 So these primers were
considered as very much useful for characterizing tungro resistant genotypes The
amplicons size of RM2 l (I 86 bp) was found in tungro susceptible. BR 10 and
moderately resistant genotypes while that was absent in highly resistant and
susceptible, BRI I genotypes. In Vlx'I'R primer GF, two amplicons {543 bp and 1003
bp) were fixed with susceptible and moderately resistant varieties while those were
absent in highly tungro resistant vaneties. Based on Nei's genetic distance, seven
clusters were formed among the tungro resistant genotypes Diversity analysis of
genotypes using morphological and molecular data indicated that new breeding
program could be initiated preferably using the parents. BRRl dhan44 and
Kachamota, BRRI dhan32 and Kacharnota, BR l 0 and Kachamota, BRRl dhan3 1 and
Kacharnota, BRRI dhan32 and Sonahidernota for the development oftungro resistant
varieties.
