Abstract:
This study was undertaken to introgress cold tolerance from IR90688-20-1-1-1-11
which showed strong cold tolerance under Bangladesh condition into BRRI
dhan28 using marker assisted backcross (MABC) technique. The experiment was
conducted in the Molecular Biology Laboratory and experimental field of
Bangladesh Rice Research Institute (BRRI) during the period of April 2015 to
October 2016. This technique was powered by phenotypic selection and screening
of seedling stage cold tolerance. A total of 47 polymorphic markers distributed at
an average distance of 18.8 cM over the genome and showing distinct difference
in band position were used in background genotyping of 41 BC1F1 plants very
close to BRRI dhan28 in phenotype. In total, nine plants with RPG value 60% or
more from the BC1 cycle were subjected to screening against cold stress at
seedling stage and finally BC2F1 progenies of two plants (BC1-239 and BC1-265)
having LD values ≤5, RPG (≥70%) and number of chromosomes with 75% to
100% R alleles were selected to proceed further for next backcross cycle.
Description:
A Thesis
submitted to the Faculty of Agriculture,
Sher-e-Bangla Agricultural University, Dhaka,
in partial fulfillment of the requirements
for the degree of
MASTER OF SCIENCE
IN
GENETICS AND PLANT BREEDING
SEMESTER: JULY-DECEMBER, 2015
