Abstract:
The present study was undertaken in the Genetics and Plant Breeding Laboratory
and the Tissue Culture Laboratory of the Department of Biotechnology. Sher-e-
Bangla Agricultural University (SAU), Dhaka, during the period of January 2013
to March 2014 , to investigate the in vitro regeneration potentiality of three potato
cultivars viz. Diamant, Cardinal. and Granola under different chemical mutagen
treatment. Five tevels of GA3 (100, 200, 300,400 and 500 ppmIL) were used to
find out the best concentration for sprout initiation . It was revealed that 500
ppmlL GA3 showed maximum sprouting within short period of time. The effect of
concentration of 2,4-I) EMS and 5-RU on in vitro callus induction and plant
regeneration were studied on I mg/L, 3mg/L, 5mg/Id concentration. Only 2, 4-D
had the ability to induce callus. The highest callus size ( 2.30 cm) was observed at
MS+3A0 mgfL 2,4-D with the variety Granola. The maximum number of shoot
(13.67) were found in the treatment MS-F3.0 mgfL 2,4-D in the variety Granola at
45 DAI and the highest number of root (14.0) were also found in the same
treatment. The highest number of shoot/plantlet (7.30) was recorded in variety
Granola at MS + 1.0 mg/L EMS and the highest number of leaves/plantlet were
also recorded in the same variety (17.30 leaves/plantlet) at the concentration of
MS+3.0 mgfL at 45 DA!. The highest number of roots/plantlet was noted in the
variety Granola (11.00 roots/plantlet) at normal MS medium at 45 DAL The
higher concentration (5.0 mg/I) of EMS and RU showed huge abnormality on in
vitro regeneration in all three varieties of potato. Thin stem, deformed shoot
development and very less leaf formation were also observed in higher
concentration. The mutagen treated variants were acclimatized in plastic tray and
subsequently in the field condition. It was noticed that, the variety Granola
survived at the high rate in plastic tray and in open atmosphere . Among all the
varieties Granola showed the best performance on callus induction plant
regeneration and in vivo establishment under field condition.The mutagenic treated
minituber were harvested and preserved for further study.
Description:
A Thesis
Submitted to the Faculty of Agriculture
Sher-e-Bangla Agricultural University, Dhaka,
in partial fulfillment of the requirements
for the degree of
MASTER OF SCIENCE
IN
GENETICS AND PLANT BREEDING
SEMESTER: JANUARY-JUNE, 2014
