http://localhost:8080/handle/123456789/5039 Sat, 18 Apr 2026 21:14:54 GMT 2026-04-18T21:14:54Z http://localhost:8080/handle/123456789/5044 SPECIES IDENTIFICATION AND MITOCHONDRIAL GENOME SEQUENCING OF FEATHERBACK CHITAL FISH (Chitala chitala) AKTER, MAHMUDA The purpose of this study was to characterize the mitochondrial genome sequencing of Chitala chitala. The complete mitogenome of C. chitala was 16248 bp long and contains 37 mitochondrial genes, including 13 typical protein coding genes, 22 tRNA genes, two ribosomal RNAs (12SrRNA and 16SrRNA), and two non-coding areas (control region, D-loop, and origin of light strand, OL). The heavy (H) strand encoded 28 genes, while the light (L) strand encoded the remaining 9 genes. A total of 31 bp of overlapped area was discovered across the C. chitala mitogenome in 13 distinct places. The mitogenome contained six intergenic spacers totaling 24 bp in length. The longest spacer was an 8-bp nucleotide sequence located between the tRNA and ND1 genes. The 13 protein-coding genes (PCGs) were 11,423 bp in length and accounted for 70.30% of the mitogenome. The base composition was 25.16% T, 29.84% C, 31.4% A, and 13.5% G. C. chitala's circular genome had a short subunit of rRNA (12S rRNA) and a large subunit of rRNA (16S rRNA), both of which were 956 bp and 1702 bp in length, respectively. The 12S rRNA gene had an overall base composition of A = 32.74%, T = 21.75%, C = 25%, and G = 20.50%, while the 16S rRNA gene had an overall base composition of A = 36.13%, T = 19.03%, C = 24.61%, and G = 20.21%. tRNA genes ranged in length from 67 to 76 bp, for a total length of 1,570 bp (9.6% of the total mitogenome). Fourteen tRNA genes were transcribed on the H-strand, while the remaining eight tRNA genes were transcribed on the L-strand. C. chitala's largest non-coding region (control region) consists of 572 nucleotides, accounting for 3.5% of the total mitogenome. The morphologically detected fish species shared 99% of their DNA with C. ornate (Accession No. AP008923.1). Since there is no verified complete mitogenome of Chitala chitala. Currently, it has been considering as a provisional reference sequence (NC_070068.1). A Thesis Submitted to the Faculty of Agriculture, Sher-e-Bangla Agricultural University, Dhaka, in partial fulfillment of the requirements for the degree of MASTERS OF SCIENCE IN BIOTECHNOLOGY Sat, 01 Jan 2022 00:00:00 GMT http://localhost:8080/handle/123456789/5044 2022-01-01T00:00:00Z http://localhost:8080/handle/123456789/5043 DNA FINGERPRINTING AND MOLECULAR DIVERSITY OF MUNG BEAN (Vigna radiata (L.) R. Wilczek) GERMPLASM TAWFIQ, TAMANNA Mung bean (Vigna radiata (L.) R. Wilczek) has been widely used as a pulse crop. The study was carried out at the Biotechnology laboratory of Sher-e-Bangla Agricultural University, during the period of July 2021 to June 2022 to investigate molecular diversity and fingerprinting of fifteen mung bean germplasm. RAPD primers were selected and synthesized for molecular diversity analysis. All of the primers were able to produce polymorphic bands. The highest number of bands were observed in RAPD primer OPT20. The polymorphic Information Content (PIC) of RAPD markers ranged from 0.24 to 0.32 with an average value of PIC =0.27. Gene diversity ranges from 0.41 to 0.28 with an average value of 0.33. Primer showed OPBC-1, OPA-20, OPP-03, OPT-20 moderate diversity and primer OPBC 18 showed low diversity on the basis of PIC value. The value of pair -wise comparisons of Nei’s (1983) genetic distance (D) among 15 genotypes was computed from and it was ranged from 0.1034 to 0.6207 with an average of 0.3621. Dendrogram based on Nei’s genetic distance using unweighted Pair Group Method Of Arithmetic Mean (UPGMA) indicated the segregation of 15 genotypes into four main clusters. This result can be conveniently used for molecular diversity analysis to identify diverse parent for mung bean improvement program. A Thesis Submitted to the Faculty of Agriculture, Sher-e-Bangla Agricultural University, Dhaka, in partial fulfillment of the requirements For the degree of MASTER OF SCIENCE IN BIOTECHNOLOGY Sat, 01 Jan 2022 00:00:00 GMT http://localhost:8080/handle/123456789/5043 2022-01-01T00:00:00Z http://localhost:8080/handle/123456789/5042 IN VITRO REGENERATION OF Aloe vera L. IN A NEW PLANT TISSUE CULTURE MEDIUM SARKER, CHHANDA The experiment was conducted at the Biotechnology Laboratory, Department of Biotechnology, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh during the period from July 2021 to June 2022, to evaluate the regeneration of Aloe vera in a new tissue culture medium (SAU Tissue Culture Medium or Hoque medium) which was marked as Treatment-3. MS (1962) medium was used as Treatment-1 and MS Powder (Duchefa, The Netharland) was used as Treatment2. It was observed that, the highest percent (90%) of shoot regeneration was observed in the SAU Tissue Culture Medium. The same treatment showed maximum number of shoot in all the study period. The highest number of leaves were found in MS powder (Duchefa, The Netherland) medium and it was lowest in MS (1962) medium. Longest length of root (7.00) cm was observed in SAU Tissue Culture Medium and it was shortage (5.10) cm in MS (1962) Medium. Those three treatments were supplemented with same dose of phytohormone (1.00mg/L KIN + 2.00 mg/L NAA) to find out the ability of media in respect of hormonal condition. It showed that there was no significant difference in respect of days to shoot initiation and percent of shoot regeneration among the SAU Tissue Culture Medium and MS powder (Duchefa, The Netharland) when supllimented with phytohormone. Number of shoot per explant and number of leaves also showed non-significant difference between the Treatment-3 (SAU Tissue Culture Medium) and Treatment-2{MS powder (Duchefa, The Netherland)}. Percent of root induction and length of root also showed similar result among the Treatment-2 and Treatment-3. The MS (1962) medium showed lowest result for all the parameter under studied. The study highlighted the potentiality of SAU Tissue Culture Medium on In vitro regeneration of Aloe vera. It has indicated that the newly developed SAU Tissue Culture Medium (Hoque Medium) has excellent result on plantlet regeneration in Aloe vera. Hence, the formulation of SAU Tissue Culture Medium is a successful innovation in the field of tissue culture technology which can be applied for any other plant species. A thesis Paper Submitted to the faculty of Agriculture, Sher-e-Bangla Agricultural University, Dhaka in partial fulfillment of the requirements for the degree of MASTER OF SCIENCE Sat, 01 Jan 2022 00:00:00 GMT http://localhost:8080/handle/123456789/5042 2022-01-01T00:00:00Z http://localhost:8080/handle/123456789/5041 MICROPROPAGATION OF SWEET POTATO (Ipomoea batatas L.) IN A NEW PLANT TISSUE CULTURE MEDIUM SULTAN, TIPU The experiment was conducted at the Biotechnology Laboratory, Department of Biotechnology, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh during the period of January 2021 to December 2021, to study the micro propagation of sweet potato (Ipomoea batatas L.) in a new plant tissue culture medium (SAU tissue culture medium). Six different culture media were compared to explore the potential of SAU Tissue cult ure medium for regeneration of sweet potato. Data were recorded on the percentage of regeneration, days to shoot initiation, and the number of shoots per explant at different time intervals. The SAU tissue culture medium showed best performance for all the parameter under study among the two other treatments MS (1962) medium and MS powder medium. The results showed that SAU Tissue Culture Medium supplemented with 1.00 mg/L KIN+2.00 mg/L NAA had the highest percentage of regeneration (90%), shorter days to shoot initiation (12.33 days) as compared to other treatments. The highest number of leaves per explant (11.33) and maximum length of shoot (9.2cm) were also observed in the same medium at 35 DAI. In regenerated plantlets 90% survival was found in growth chamber, 85% survival under shade house and 80% in open field condition. Micropropagation of sweet potato was successfully done in SAU tissue culture medium which can be utilized for large scale plantlet production program. A Thesis Submitted to the faculty of Agriculture, Sher-e-Bangla Agricultural University, Dhaka for partial fulfillment of the requirements for the degree of MASTER OF SCIENCE (MS) IN BIOTECHNOLOGY Sat, 01 Jan 2022 00:00:00 GMT http://localhost:8080/handle/123456789/5041 2022-01-01T00:00:00Z