http://localhost:8080/handle/123456789/2771 Sat, 18 Apr 2026 21:03:14 GMT 2026-04-18T21:03:14Z http://localhost:8080/handle/123456789/760 INTRODUCTION OF SALT TOLERANT GENE IN WHITE JUTE THROUGH AGROBACTERIUM VECTOR AMIN, MD. NURUL An experiment was conducted at the Biotechnology Laboratory, Department of Genetic Resources and Seed Division. IIangladcsh Jute Research Institute. Dhaka during the period of march 2008 to February 2009 on white Jute (Corchorus capsulari.c) which is one of the world's most economical!)' important libcr crops. Genetic Engineering or recombinant DNA technology can resolve the various biotic and abiotic problems by making transgenic plant. The regeneration and transtbrmation processes depend on optimum growth conditions, suitable explants and varieties. An attempt was made for Agrobaclenum mediated genetic transformation in white jute varieties using gene construct conferring both salt and drought tolerance (CIPK and Glv-l)along with the marker genes. Interestingly the three varieties (CVL-1.CVF-3 and Tricap- I) showed the response of both callus induction and plant regeneration on a single formulation i.e. MS medium supplemented with 2.0 mg/I BAP and 0.5 mg/I IAA and cotyledons with attach petioles of 7-8 days old seedling .An efficient and reproducible protocol for transgenic jute plants was established by inoculating cotyledons with attached petioles and hypocotile with agrobcwierium turnefaciens strains LBA4404contains both salt and drought tolerant genes (CIPK and (fly-I with selectable marker gene Nptll conferring resistance to kanamycin and GUS reporter gene also. Explants were dipped to liquid culture of bacteria for one minute and then transferred to co-cultivation media for 24 hours. Shoot regeneration from Agrohacieriwn infected cotyledon was fotind highest in variety CVI.-1 than Trieap. After co-cultivation and selection histoehemieal GUS assay was performed in three varieties (vars. Tricap-l. CVL-1 and CVE-3). In the transformed explants. GUS reporter gene was expressed showing blue color in the explant tissues. Among the varieties CVE-3 Showed the highest expression blue color in the explant tissues. l'hose transgenic plants are transferred to salt medium and soil for trial. A Thesis Submitted to the Faculty of Agriculture, Sher-e-Bangla Agricultural University, Dhaka, in partial fulfillment of the requirements for the degree of MASTER OF SCIENCE IN GENETICS AND PLANT BREEDING SEMESTER: JANUARY-JUNE, 2009 Mon, 01 Jun 2009 00:00:00 GMT http://localhost:8080/handle/123456789/760 2009-06-01T00:00:00Z http://localhost:8080/handle/123456789/758 GENETIC DIVERGENCE, VARIABILITY, CORRELATION AND PATH ANALYSIS IN WHITE JUTE (Core/torus eapsularis L) ALAM, MUHAMMAD JAHANGIR The experiment was conducted with fifty one genotypes of white jute from different geographic origin were evaluated to study their genetic divergence, variability, correlation and path analysis with 11 morphological characters. The experiment was carried out at the Central Jute Research Experiment Station of Bangladesh Jute Research Institute (BJRL), Jagir, Manikgonj during the period from April to August, 2010. Analysis of variance revealed significant variation among the genotypes for all the characters. Multivariate techniques were used to classify 51 genotypes, which computed by MahalanobisD2statistics. All the genotypes were grouped into six different clusters. Principal component analysis, principal coordinate analysis, canonical variate analysis and cluster analysis gave similar results. Cluster V and VI had the maximum ten genotypes while cluster I had the minimum of seven genotypes. The highest inter-genotypic distance (1.8441) was found between 015, (iso and the lowest distance between Gg and 026 . The highest inter-cluster distance (14.367) was observed between cluster I, IV and the lowest distance (2.458) was found between cluster Ill and V. The highest intra-cluster distance was found in cluster I and lowest in cluster V. Considering genetic parameters, high genotypic coefficient of variation (CICV) was observed for branches per plant. High heritability values with moderate genetic advance in percentage of mean were obtained for leaf width, petiole length, nodes per plant. Correlation studies showed positive correlation between fibre yield and its most components. Path analysis showed highest positive direct effect of stick weight on fibre weight followed by base diameter, leaf width and petiole length. Considering the cluster distance, inter-genotypic distance and other agronomic performance, the genotypes 047,033, 048 from cluster 1; 027, 017, 023 from cluster Ill and G13,G40,045 from cluster II were considered to be better parents for future use in hybridization programme. A Thesis Submitted to the faculty of Agriculture, Sher-e-Bangla Agricultural University, Dhaka in partial fulfilment of the requirements for the degree of MASTER OF SCiENCE IN GENETICS AND PLANT BREEDiNG SEMESTER: JULY-DECEMBER, 2009 Tue, 01 Dec 2009 00:00:00 GMT http://localhost:8080/handle/123456789/758 2009-12-01T00:00:00Z http://localhost:8080/handle/123456789/755 GLADIOLUS (Gladiolus grandiflorus L.) SCENARIO AND ITS IMPROVEMENT THROUGH INTERVARIETAL HYBRIDIZATION IN BANGLADESH HOSSAIN, MD. DELUAR The present experiment was carried out during the period from November'2008 to October' 2009 to investigate the morphology, market demand. and production of hybrid seed and % of hybrid seed germination in gladiolus. The experiment included five gladiolus genotypes. The results indicated the existence of wide variability among the genotypes on their morphological characters along with yield and yield attributes. Genotypes varied from 59.63 cm to 92.05 cm and 25.66 cm to 47.30cm in spike length and raehis length, respectively. Number of flower was the highest in white (14.25) and the lowest in orange (8.40) genotype. Number of connel and eorrnel weight of the genotypes significantly difThred and ranged from 25.70 to 43.20 and 5.20g to 6.40 g. respectively. The price of gladiolus flower varied from genotype to genotype and the demand and price of gladiolus flower also varied in different month of the year, different clays of the week and different special days of the year. Considering crossing parameters, variation were observed for length of capsule (cm). breadth of capsule and thousand seed weight (g). Mean data indicated that all the crosses produced gladiolus true seed i.e. F1 seed. The number of seeds per capsule ranged from 15.08 to 38.40. The highest number of seed per capsule was produced by the cross Yellow x Red (38.40) while the lowest number of seed per capsule was produced by the cross Violet x Red (15.08). A Thesis Submitted to the Faculty of Agriculture, Sher-e-Bangla Agricultural University, Dhaka, in partial fulfillment of the requirements for the degree of MASTER OF SCIENCE IN GENETICS AND PLANT BREEDING SEMESTER: JANUARY- .JUNE, 2009 Mon, 01 Jun 2009 00:00:00 GMT http://localhost:8080/handle/123456789/755 2009-06-01T00:00:00Z http://localhost:8080/handle/123456789/751 GENETIC VARIABILITY AND CHARACTER ASSOCIATION IN CHICK PEA (Cicer arietinum L) SYED, MD. ABU An experiment was conducted to study genetic variability, character association, genetic divergence and path coefficient analysis for yield and yield contributing traits in chick pea. The study was carried out at the experimental farm, Department of Genetics and Plant Breeding, Sher-e-Bangla Agricultural University, Dhaka, during October 2008 to April 2009. The results indicated that different chick pea genotypes differed significantly regarding all the characters studied. Minimum differences of genotypic and phenotypic variances and coefficient of variation as well as high heritability coupled with high genetic advance and genetic advanced in percent of mean were observed for all the traits of different chick pea genotypes indicated additive gene effects of these traits. Number of pods per plant and 100 seeds weight of different genotypes had a high degree of significant positive association with seed yield per plant and high positive direct effect indicated that these characters had the major contribution towards the grain yield per hill. Selection could be made based on two traits for the improvement of yield of chick pea. Cluster H comprised maximum number of (11) genotypes followed by cluster Ill, V. IV and 1. The highest inter genotypic distance was observed between the genotype BD 6549 and 130 6603 (2.09). The inter cluster distance was maximum between the clusters I and Il (16.35). Among the characters number of secondary branches (0.11) and seed yield per plant (0.01) were major characters that contributed mostly toward genetic divergence. A Thesis Submitted to the Faculty of Agriculture, Sher-e-Bangla Agricultural University, Dhaka, in partial fulfillment of the requirements for the degree of MASTER OF SCIENCE IN GENETICS AND PLANT BREEDING SEMESTER: JANUARY- JUNE, 2009 Mon, 01 Jun 2009 00:00:00 GMT http://localhost:8080/handle/123456789/751 2009-06-01T00:00:00Z