Year 2016
http://archive.saulibrary.edu.bd:8080/xmlui/handle/123456789/2818
2024-03-29T12:17:51ZIN VITRO PROPAGATION OF GARLIC (Allium sativum L.)
http://archive.saulibrary.edu.bd:8080/xmlui/handle/123456789/414
IN VITRO PROPAGATION OF GARLIC (Allium sativum L.)
MUBARRAT, FABEEHA
The research work was conducted in the Laboratory of Biotechnology of the Department of
Biotechnology, Sher-e-Bangla Agricultural University, from the period of July 2015 to June,
2016 to observe the response of different plant growth regulators on in vitro regeneration of
garlic. The disease free, healthy and sterilized basal part of clove of 2-3 cm length were used
as explants. Sterilization was done with 70% ethanol, 0.5% HgCl
2
along with few drops of
Tween-20. Explants were placed in MS medium supplemented with the Kinetin (KIN), alone
and in combination with 2, 4-Dichlorophenoxy acetic acid (2, 4-D). The highest shoot
regeneration percentage (100%) was observed by 3.0 mg/L of KIN alone and by wide range
of KIN in combination with 2, 4-D (i.e. all combination of 2.5, 3.0 mg/L KIN with 1.0, 1.5,
2.0, 2.5 mg/L 2, 4-D) . The highest shoot length (34.50 cm) was observed after 3 weeks of
initiation in 3.0 mg/L KIN treatment. Combined doses of KIN and 2, 4-D showed 1 shoot
after 3 weeks of initiation. Number of leaves did not exceed 3 and it was noticed through all
combination of 2.0, 2.5, 3.0 mg/L KIN with 1.0, 1.5, 2.0, 2.5 mg/L of 2, 4-D. The highest
root induction (100%) was observed by 2.5 mg/L KIN + (1.0, 1.5) mg/L 2, 4-D. It took
minimum 3 days to induce root. The average and the highest length of root (3.5 cm and 2.93
cm respectively) was noticed by application of 3 mg/L KIN and 1.0 mg/L 2, 4-D. Maximum
number of roots (10.67) were developed by 3.0 mg/L KIN+ 1.5 mg/L 2, 4-D. After
transferring the plantlets in the field condition, 86% survival plants were recorded. Finally, in
vitro regeneration of garlic was proved to be less time consuming. Moderate doses of
hormones showed satisfactory responses in shoot proliferation and root induction which
made the tool cost effective as well.
A Thesis
Submitted to the Faculty of Agriculture,
Sher-e-Bangla Agricultural University, Dhaka,
in partial fulfillment of the requirements
for the degree of
MASTER OF SCIENCE
IN
BIOTECHNOLOGY
SEMESTER: JANUARY-JUNE, 2016
2016-12-01T00:00:00ZPHENOTYPIC CHARACTERIZATION AND GENETIC DIVERSITY ANALYSIS OF EGGPLANT (Solanum spp.) GERMPLASM USING SSR MARKER
http://archive.saulibrary.edu.bd:8080/xmlui/handle/123456789/413
PHENOTYPIC CHARACTERIZATION AND GENETIC DIVERSITY ANALYSIS OF EGGPLANT (Solanum spp.) GERMPLASM USING SSR MARKER
TASRIN, KASHPIA
An experiment was carried out with twenty five local and two wild relatives (Solanum
sisymbriifolium and S. torvum) of eggplant at the research field and in the
Biotechnology Laboratory of Sher-e-Bangla Agricultural University, Dhaka,
Bangladesh, during October 2015 to June 2016 to investigate the phenotypic
characterization and molecular diversity analysis of different local eggplant
germplasm and its wild relatives. The morphological study revealed diversified
characters among all the genotypes. Variations were observed in color of leaf vein,
stem as well as fruit size and shape. Maximum yield per plant was recorded in Salta
Begun and it was 9.8 kg. The minimum yield per plant was observed in both the wild
species (3.8 kg) as they produced very small fruits. Correlation of yield contributing
traits separated all the genotypes into four clusters (A, B, C, and D). Among them
cluster D had five genotypes together with the wild species indicated their relatedness.
Five well-known SSR primers were used for the molecular characterization of the
genotypes. Ten alleles, ranged from 1 to 3 alleles per locus and an average of 2.0 were
detected. Among five primers three were able to produce polymorphic bands and the
total number of polymorphic bands was five. The highest (2) number of bands was
observed in SSR primers EPSSR82 and smSSR01. The Polymorphism Information
Content (PIC) of SSR markers ranged from 0.37 (smSSR01) to 0.67 (EPSSRR82)
with an average value of PIC = 0.54. Gene diversity ranges from 0.49 (smSSR01) to
0.72 (EPSSR82), with an average value of 0.61. The value of pair-wise comparisons
of Nei‟s (1972) genetic distance (D) and identity between varieties was computed
from combined data for the five primers, ranged from 0.20 to 1.00, with an average
value of 0.60. Multivariate cluster analysis on the basis of Nei‟s genet ic distance and
identity revealed that within those 27 genotypes there surely do exist considerable
diversity. UPGMA method separated the segregation of 27 genotypes into two major
clusters (I and II). From the clusters, wild species Solanum torvum belonged to the
sub-cluster (IIb), that revealed its distinct variation from the others. On the other hand,
wild species Solanum sisymbriifolium showed a close relatedness by forming the
same cluster together with thirteen local eggplant genotypes. This experiment brought
a great opportunity for eggplant improvement as the local genotypes had a close
relation with the wild species.
A Thesis Submitted to
The Department of Biotechnology, Faculty of Agriculture
Sher-e-Bangla Agricultural University, Dhaka-1207
In partial fulfillment of the requirements
For the degree
Of
MASTER OF SCIENCE (MS)
IN
BIOTECHNOLOGY
SEMESTER: JANUARY-JUNE, 2016
2016-12-01T00:00:00ZRAPID GENOMIC DNA EXTRACTION IN BULB CROPS AND ESTIMATION OF GENETIC DIVERSITY IN GARLIC (Allium sativumL.) USING RAPD MARKER
http://archive.saulibrary.edu.bd:8080/xmlui/handle/123456789/412
RAPID GENOMIC DNA EXTRACTION IN BULB CROPS AND ESTIMATION OF GENETIC DIVERSITY IN GARLIC (Allium sativumL.) USING RAPD MARKER
SHEULY, KAMRUN NAHAR
An experiment was carried out in Biotechnology Laboratory of the Department of
Biotechnology, Sher-e-Bangla Agricultural University (SAU), Dhaka, Bangladesh
to develop low cost and rapid genomic DNA isolation technique and to study the
DNA fingerprinting and genetic diversity of nine garlic genotypes. Conventional
CTAB/SDS method of DNA extraction required more time and cost. In contrast,
the newly developed method to low cost and very fast method for extraction of
genomic DNA in bulb crops. The garlic germplasms were BARI-G1, GC001,
GC008, GC0012, GC0013, GC0017, GC0024, GC0027 and GC0028. Genomic
DNA was extracted in both methods to perform PCR reaction with seven RAPD
primers. Sum of 53 distinct and differential amplified DNA bands were generated
from PCR amplification. Out of them, 40 bands (75.88%) were polymorphic and
13 bands (24.12%) were monomorphic. The Nei’s genetic identity among 9 garlic
genotypes ranged from 0.8053 to 0.9966 and genetic distance ranged from 0.0053
to 0.2166. The UPGMA Dendrogram segregated the 9 garlic genotypes into two
main clusters. The first cluster contained 1 genotypes and the second cluster had 8
genotypes. The Dendrogram also indicated that BARI-G1 vs GC0027 varietal pair
showed highest Nei’s genetic distance (0.2166) and GC0012 vs GC0017 varietal
pair showed lowest genetic distance (0.0053). The study revealed a significant
amount of relationship and genetic diversity among the studied 9 garlic genotypes.
The RAPD markers were found to be useful tool for molecular characterization
and polymorphism study in garlic genotypes.
A Thesis
Submitted to the Faculty of Agriculture,
Sher-e-Bangla Agricultural University, Dhaka
In partial fulfillment of the requirements
For the degree of
MASTER OF SCIENCE
IN
BIOTECHNOLOGY
SEMESTER: January - June, 2016
2016-12-01T00:00:00ZMICROPROPAGATION OF SALT TOLERANT EXOTIC POTATO GENOTYPES AND THEIR IN VITRO BIOASSAY
http://archive.saulibrary.edu.bd:8080/xmlui/handle/123456789/411
MICROPROPAGATION OF SALT TOLERANT EXOTIC POTATO GENOTYPES AND THEIR IN VITRO BIOASSAY
RAHMAN, MD. HABIBUR
In vitro bioassay of nine exotic potato genotypes namely CIP102, CIP106, CIP111, CIP
117, CIP 120, CIP124, CIP127, CIP 136 and CIP 139 was conducted for salinity
tolerance at Tissue Culture Lab, Tuber Crops Research Center (TCRC), Bangladesh
Agricultural Research Institute (BARI), Gazipur-1701. Single node and root tip
segments of these genotypes were cultured in MS media supplemented with 0.0
(control), 80, 100, 120, 140 and 160 mM NaCl. In case of in vitro shoot bioassay, CIP
139 was found as the most salt tolerant with the highest plant height (9.67 cm), number
of nodes (9.50), number of leaves (13.60), number of roots (8.00), length of root (6.50
cm), fresh weight of shoot (509.0 mg) and fresh weight of root (205.60 mg) at 160 mM
NaCl (14.61 dSm
). On the other hand, CIP 106 was found as the most salinity
sensitive at 120 mM NaCl (10.96 dSm
-1
) producing minimum plant height (7.17 cm),
number of nodes (6.50), number of leaves (12.50), number of roots (9.70), length of
roots (5.10 cm), fresh weight of shoot (572.3 mg) and fresh weight of root (244.4 mg)
followed by CIP 136, CIP 117 and CIP 111 at same salinity level. However, CIP 127,
CIP 102 and CIP 124 genotypes showed very good performance up to 140 mM NaCl
(12.78 dSm
-1
-1
). In vitro root bioassay also revealed the highest salinity tolerance of CIP
139 up to 160 mM NaCl, CIP 127 and CIP 102 up to 140 mM NaCl as well as CIP 106
up to 120 mM salinity level. The root tips of experimented potato genotypes were not
significantly affected up to 120 mM salinity level in comparison with control where CIP
120 was found as the lowest tolerant up to 120 mM salinity level in MS media. Among
the 0.0 (control), 0.1, 0.5, 1.0 and 1.5 mg /L IBA concentrations supplemented with MS
media 1.0 mg/ L was found best for rooting with early root initiation (6.57 days) and
well development (10.48 days), highest root length (8.63 cm), 2
highest root number
(17.25) and maximum fresh weight of root (332.22 mg). Interestingly, there was no
significant differences between 0.5 and 1.0 mg/L IBA for in vitro root induction and
development in the experimented CIP potato genotypes.
A Thesis
Submitted to the Faculty of Agriculture,
Sher-e-Bangla Agricultural University, Dhaka,
in partial fulfilment of the requirements
for the degree of
MASTER OF SCIENCE
IN
BIOTECHNOLOGY
Semester: January-June 2016
2016-12-01T00:00:00Z