http://localhost:8080/handle/123456789/2775 2026-04-04T13:31:37Z http://localhost:8080/handle/123456789/825 GENERATING DOUBLED HAPLOID MUSTARD (Brasska rapa L.) PLANTS THROUGH ANTHER CULTURE HALIM, MD. ABDUL The present study was undertaken in the Tissue Culture Laboratory of the Department of Biotechnology. Sher-e-Bangla Agricultural University. Shcr-e-Bangla Nagar, Dhaka- 1207, during the period of August 2010 to March 2012 generated calli. shooting through anther cultuc for doubled haploid ci' mustard (Brassica rapa L.) plants. There were three genotypes ofF1 generation of SAU Sharisha IX Tori 7. BARI Sharisha 15 X Tori 7 and BARI Sharisha 6 X Tori 7 and nine hormonal combination treatments were used 11w callus development and fifteen hormonal combination treatments were used for shooting under present study. Anthers were used as explants. Six different diameter of [lower buds (1.00-1.50. 1.51-2.00. 2.01-2.50. 2.51-3.001, 3.01-3.50 and 3.51-4.00 mm) and six different cold pretreatment (40c) (2, 3. 4. 5. 6 and 7 days) and their combined effect was studied for callus induction. From experiment I it showed that highest percentage ( 70%) when 3.01-3.50 mm flower bud size are used in combination with 4 days cold pretreatment (4°(') in the genotype ofF1 generation of SAU Sharisha Ix Tori 7. From experiment 2 it showed that the minimum (4.2006 days) days to callus induction at T5 4.0 mg/f. 2. 4-D 4.0 mg/L BAP) in genotype of F 1 generation SAU Sharisha I x Tori7. The maximum breadth (1.520 cm at 35 DAI), length (1.920 em at 35 DAI) and size (1.720 cm at 35 DAI) was observed in combination of T7(6.0 mg/L 2. 4-D2.0 mg/L BAP) and the genotype F1 generation SAU Sharisha I x Tori7. The maximum (0.7020 and 1.302 gm at 14 and 35 DAI respectively) callus weight was observed in combination l'7(6.0 mg/L 2, 4-D±2.0 nigfL BAP) and the genotype oiF1 generation of BARI Sharisha 6 x Tori 7. The minimum days to shoot initiation (4.20days) was observed in the genotype ofF1 of BARI Sharisha 15 x Tori 7 with T12 (1.0 BAP + 0.5 2,4-D). A Thesis Submitted to the Faculty of Agriculture, Sher-e-Bangla Agricultural University, Dhaka, in partial fulfillment of the requirements for the degree of MASTERS OF SCIENCE IN GENETICS AND PLANT BREEDING SEMESTER: JANUARY-JUNE, 2011 2011-06-01T00:00:00Z http://localhost:8080/handle/123456789/824 GENETIC DIVERSITY ANALYSIS IN SOYBEAN (Glycine max (L.) Merrill) AKTER, MOST. NAJMUNNAIIAR TASRIFA A field experiment was conducted during November 2011 to March 2012 to study the genetic diversity for quantitative traits in soybean (Glycinc may L. Merrill) with 25 genotypes in randomized block design with three replications. The genotypes were placed in a field experiment conducted at the research farm of Sher-e-l3angla Agricultural University, Dhaka- 1207. The objectives of the study were to asses the genetic diversity among the genotypes of soybean, to find out the characters influencing genetic divergence, to estimate heritability and genetic advance of yield and yield components, to screen out the parental groups which are likely to provide superior segregants on hybridization and to know the yield potentiality of genotypes. Analysis of variance for each trait showed significant differences among the genotypes. Phenotypic coefficients of variation (PCV) was also close to genotypic coefficients of variation (GCV) for all the characters except branches per plant, pod length and seeds per pod, effective node number indicating that environment had influence on the expression of these characters. High heritability associated with high genetic advance percent of mean was observed for pod length and hundred seed weight which indicated that selection for these characters would be effective. Hence, thrust has to be given for these characters in future breeding programme to improve the yield in soybean. Multivariate analysis based on 13 agronomic characters indicated that the 25 genotypes were grouped into five distant clusters. The maximum contribution of characters towards diversity was observed by days to maturity, seeds per pod and seed yield per plant. Thus, these traits may be given high emphasis while selecting the lines for hybridization. The inter cluster distance was maximum between cluster I and cluster IV. The highest intra-cluster distance was found in cluster IT. From the results it can be concluded that the following genotypes vi:., G1r. (BD-2339), G12 (RD-2335), G13 (BD-2336), and GJ4 (BD-2337) were identified as potential genotypes for higher seed yield in soybean. A thesis Submitted to the Faculty of Agriculture, Sher-e-Bangla Agricultural University, Dhaka. in pail/a/fulfilment of the requirements for the degree of MASTER OF SCIENCE IN GENETICS AND PLANT BREEDING SEMESTER: JANUARY- JUNE, 2011 2011-06-01T00:00:00Z http://localhost:8080/handle/123456789/823 GENETIC DIVERGENCE ANALYSIS IN LENTIL (Leiss culinaries Medik.) HOSSEN, FAKIR SOHAG A field experiment was conducted with 35 lentil genotypes at Sher-e-Bangla Agricultural University experimental farm, Sher-e-Bangla Nagar. Dhaka, to study their diversity based on different morphological characteristics during November 2011 to March 2012. The objectives of the study were to identify divergent parents for hybridization program, to identify the characters contributing to genetic diversity, to assess the magnitude of genetic divergence in genotypes, association among the characters and their contribution to yield. The analysis of variance indicated significantly higher amount of variability among the genotypes for all the characters. Different multivariate analysis techniques were used to classi 35 lentil genotypes. Diversity was estimated by cluster distance. All the genotypes were grouped into five clusters. Principal Component Analysis, Cluster Analysis and Canonical Variate Analysis exhibited similar results. Significant variations were observed among the lentil genotypes for all the parameters under study. Cluster lii had the maximum (13) and cluster II had the minimum (1) number of genotypes. The highest intra-cluster distance was observed in cluster V followed by 1. The highest inter-cluster distance was observed between cluster II and IV and the lowest inter-cluster distance was found between the clusters Ill and IV. Considering genetic parameters high genotypic co-efficient of variation (GCV) was observed for number of primary and secondary branches. number of pods per plant, seeds per plant and yield per plant whereas days to 50 % flowering, and days to 100% flowering showed low GCV. In all cases, phenotypic variances were higher than the genotypic variance. Fligh heritability with low genetic advance in percent of mean was observed for days to 100% flowering, number of pods per plant, seeds per plant, and yield per plant which indicated that non-additive gene effects were involved for the expression of this character and selection for such trait might not be rewarding. High heritability with high genetic advance in percent of mean was observed for number of pods per plant and seeds per plant indicating that this trait was under additive gene control and selection for genetic improvement for this trait would be effective. Considering all the characters 01 (RARI MASOOR-l); 027 (BD-3827); 09 (BD-3805); G3 (BARI MASOOR-3): 014 (BD-381 1) can be selected for future breeding program. A Thesis Submitted to the Faculty of Agriculture, Sher-e-l3angla Agricultural University, Dhaka, in partial fulfillment of the requirements for the degree of MASTER OF SCIENCE IN GENETICS AND PLANT BREEDING SEMESTER: January - June, 2011 2011-06-01T00:00:00Z http://localhost:8080/handle/123456789/822 GENETIC DIVERSITY ANALYSIS IN TOMATO (Solan urn lycopersicon) HOSSAIN, MANJUR Thirty live genotypes of Tomato (Solanum lycoperswon) were studied in a field experiment conducted at the experimental field of Sher-e-Bangla Agricultural University. Dhaka. during November 2010 to March 2011. The objectives of the study were to identify divergent parents for hybridization programme, to identify the characters contributing to genetic diversity, to assess the magnitude of genetic divergence in genotypes, to screen out the suitable parents group which are likely to provide superior segrcgants on hybridization. The analysis of variance indicated significantly higher amount of variability among the genotypes for all the characters. Different multivariate analysis techniques were used to classify 35 tomato genotypes. Diversity was estimated by cluster distance. All the genotypes were grouped into four clusters. Principal Component Analysis. (luster Analysis and Canonical Variate Analysis exhibited similar results. Significant variations were observed among the tomato genotypes for all the parameters under study. Cluster IV had the maximum (16) and cluster 11 had the minimum (1) number of genotypes. The highest intra-cluster distance was observed in cluster Ill followed by IV. The highest inter-cluster distance was observed between cluster IL and III and the lowest inter-cluster distance was found between the clusters I and IV. Considering genetic parameters high genotypic co-efficient of variation (GCV) was observed for number of fruits per cluster, number of fruits per plant, fruit weight and fruit yield per plant whereas days to first flowering, days to 50% flowering and days to maturity showed low GCV. In all eases, phenotypic variances were higher than the genotypic variance. High heritability with low genetic advance in percent of mean was observed for days to 50% Ilowering, number of fruits per cluster, fruit length, and fruit yield per plant which indicated that non-additive gene effects were involved for the expression of this character and selection for such trait might not be rewarding. High heritability with high genetic advance in percent of mean was observed for number of fruits per plant and fruit weight indicating that this trait was under additive gene control and selection for genetic improvement for this trait would be effective. Considering all the characters G24 (BD-7761); G27 (BAR! Tomato-3); G29 (BAR! lornato-6); (i31 (BAR! Tomato-8); G33 (BAR] Tomato- Ii) can be selected for future breeding programme. A Thesis Submitted to the Faculty of Agriculture, Sher-c-Bangla Agricultural University. Dhaka, in partial fulfillment of the requirements for the degree of MASTER OF SCIENCE IN GENETiCS AND PLANT BREEDING SEMESTER: JANUARY- JUNE, 2011 2011-06-01T00:00:00Z