http://localhost:8080/handle/123456789/2726 2026-04-18T14:23:26Z http://localhost:8080/handle/123456789/4327 ISOLATION, MOLECULAR IDENTIFICATION AND SCREENING OF FUSARIUM MYCOTOXIN DON (DEOXYNIV ALENOL) DEGRADING BACTERIA IN EUROPEAN WHEAT SAMPLES JAHAN, ISRAT Mycotoxins are the toxic substances which are produced as a result of infection caused by fungi as their secondary metabolite. Major toxin producing fungi are Fusarium, Penicillium, Aspergillus and Claviceps which produces DON, ZEN, OTA, FB, AFB, citrinin and patulin. The experiment was carried out in Key Laboratory of Food Safety and Quality Control, Institute of Food Science and Technology.Chinese Academy of Agricultural Sciences, Beijing China under completely randomized design with three replications. The objectives of the experiment were the isolation, molecular identification and screening of the single bacterial colony which is responsible for the degradation of Fusarium mycotoxin deoxynivalenol (DON) in European wheat samples. Wheat samples were mixed using lysogeny broth (LB) media, PBS media and mineral salt (MM) media for isolation of bacteria using DONstar immunoaffinity column.Six Bacteria genera namely Pseudomonas spp, Agrobacterium sp, Zobellella sp, Bacillus sp., Achromobacter sp, Advenella sp and Cupriavidus sp. were isolated and identified by peR amplifying 16SrDNA gene fragment.Maximum DON degradation was recorded by mixed bacteria in wheat samples rather than the isolated single bacteria. A Thesis Submitted to the Faculty of Agriculture, Sher-e-Bangla Agricultural University, Dhaka in Partial fulfillment of the requirements for the degree of MASTER OF SCIENCE (MS) IN PLANT PATHOLOGY 2017-01-01T00:00:00Z http://localhost:8080/handle/123456789/2527 ISOLATION, MOLECULAR IDENTIFICATION AND SCREENING OF FUSARIUM MYCOTOXIN DON JAHAN, ISRAT Mycotoxins are the toxic substances which are produced as a result of infection caused by fungi as their secondary metabolite. Major toxin producing fungi are Fusarium, Penicillium, Aspergillus and Claviceps which produces DON, ZEN, OTA, FB, AFB, citrinin and patulin. The experiment was carried out in Key Laboratory of Food Safety and Quality Control, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing China under completely randomized design with three replications. The objectives of the experiment were the isolation, molecular identification and screening of the single bacterial colony which is responsible for the degradation of Fusarium mycotoxin deoxynivalenol European wheat samples. Wheat samples were mixed using lysogeny broth media, PBS media and mineral salt DONstar immunoaffinity column. Six Bacteria genera namely Pseudomonas spp, Agrobacterium sp, Zobellella sp, Bacillus sp., Achromobacter sp, Advenella sp and Cupriavidus sp. were isolated and identified by PCR amplifying 16SrDNA gene fragment. Maximum DON degradation was recorded by mixed bacteria in wheat samples rather than the isolated single bacteria. DEPARTMENT OF PLANT PATHOLOGY SHER-E-BANGLA AGRICULTURAL UNIVERSITY DHAKA-1207 2017-01-01T00:00:00Z http://localhost:8080/handle/123456789/2465 CALLUS INDUCTION AND VIRUS FREE POTATO MINITUBER PRODUCTION THROUGH MERISTEM CULTURE SERINE, SAYMA The experiment was carried out by taking the facilities of the laboratory of Biotechnology Department, SAU and the laboratories of Plant Tissue Culture, Molecular Biology and Plant Virology, Department of Plant Pathology, Sher-eBangla Agricultural University, Dhaka-1207, during the period of July, 2016 to August 2017. The experiment was conducted with a view to establish a protocol for in vitro production of virus free mini-tubers, using potato meristem as explant collected from potato sprouts of three popular potato varieties viz. , Granola, Diamant and Cardinal. Plant growth regulator GA3 (400 ppm/L) was used to assess the influence on sprouting ability. Among the selected potato cultivars, sprouting efficiency was observed at 400ppm/L GA3 treatment within short period of time in Granola variety. The effect of different combination and concentration of 2, 4-D, BAP and IBA was used along with fresh MS media to inoculate meristems of potato sprouts. Six different combinations of 2, 4-D, BAP and IBA with a control treatment were used in the experiment. The several concentrations of 2, 4-D (2, 4-D: 0.125, 0.25, 0.37, 0.50, 1.0, 2.0 mg/L) BAP (BAP: 0.5, 1.0, 1.5, 2 mg/L) and IBA (IBA: 0.25 and 1.50 mg/L respectively) in the experiment. The maximum (0.74 cm) callus size observed in Granola within a very short (2 days) period of time while treated with T 3 (0.25 IBA+0.25 2, 4-D +1.0 BAP). Granola gave the highest (9.32 cm) values in respect of length of shoot, number of shoots/plantlets (4.33) and root length (7.14 cm) along with maximum (15.00) number of leaves/plantlets whereas Cardinal gave minimum (3.10 cm) values in respect of length of shoots/plantlets, maximum (1.33) number of shoots/plantlets and number (4.00) of leaves/plantlets was found in Diamant variety. Granola showed maximum (8.44 cm) length of root while minimum (1.87 cm) length of root was found in Cardinal variety among the selected cultivars. Over all Granola showed better performances from meristem tissue culture and plants were found normal and free from potato viruses as they were tested through ELISA test. DEPARTMENT OF PLANT PATHOLOGY SHER-E-BANGLA AGRICULTURAL UNIVERSITY DHAKA-1207 2017-01-01T00:00:00Z http://localhost:8080/handle/123456789/2464 ELISA-Based Screening of Tomato Varieties against Tomato Yellow Leaf Curl Virus (TYLCV) MD KAWSER A pot experiment was conducted in net house under the Department of Plant Pathology, Sher-e-Bangla Agricultural University, Dhaka-1207, Bangladesh. The purpose of the study was to evaluate the incidence and severity level of the Tomato Yellow Leaf Curl Virus (TYLCV) and to screen the resistance/tolerance of selected tomato varieties against TYLCV through serological test (DASELISA). DAS-ELISA test was performed at tissue culture laboratory of Bangladesh Council of Scientific and Industrial Research (BCSIR).The experiment was carried out during the period October 2017 to April 2018. In total ten tomato varieties viz. BARI Tomato-2, BARI Tomato-8, BARI Tomato-11, BARI Tomato-14, BARI Tomato-15, BARI Hybrid Tomato-5, BARI Hybrid Tomato-7, BARI Hybrid Tomato-9, Ratan and Sorno Komol were selected to conduct this study. Among the selected ten tomato varieties, the highest disease incidence (100%) was recorded in 5 (five) varieties viz. BARI Tomato-11, BARI Tomato-14, BARI Tomato-15, BARI Hybrid Tomato7 and Sorno Komol and there was no incidence was found in BARI Hybrid Tomato-5. The highest disease severity was recorded in BARI Hybrid Tomato7 (70%) and there was no severity was found in BARI Hybrid Tomato-5. Although the variety BARI Hybrid Tomato-5 was not shown any kind of typical symptoms in the net house conditions when investigated on the basis of biological properties but it was found infected in DAS-ELISA test. From the above mentioned results, it can be concluded that symptomology is not the reliable method for all virus identification/detection. Different growth parameters, growth and yield attributes were also studied in this piece of research work and it was revealed that morphology, physiology, growth and yield contributing factors are significantly affected by TYLCV infection in different varieties. DEPARTMENT OF PLANT PATHOLOGY SHER-E-BANGLA AGRICULTURAL UNIVERSITY DHAKA-1207 2017-01-01T00:00:00Z