http://localhost:8080/handle/123456789/2718 2026-04-18T14:31:58Z http://localhost:8080/handle/123456789/4252 INFLUENCE OF ARBUSCULAR MYCORRHIZAL FUNGI ON GROWTH AND NUTRIENT UPTAKE OF SOME VEGETABLE ALI, MD. MOMRAZ A pot experiment was conducted in the net house of the Department of Plant Pathology, Sher-e-Bangla Agricultural University, Dhaka during the period from May 2006 to December 2006 with a view to study the role of Arbuscular Mycorrhizal (AM) fungi on growth and nutrient uptake of vegetable crops(Brinjal,Tomato,ChiJIi,Okra and Data). Significant positive growth response to AM was observed in all the selected vegetables. The seedling emergence, plant height, shoot length and root length of inoculated vegetables were comparatively higher than that of uninoculated control. Mycorrhizal fungi inoculation significantly enhanced disease reduction in all the treatment compared to control plant. In case of Okra the incidence of damping off and foot rot were )0.78% and 6.48% in noninoculated plant whereas 4.52% and 3.24% in inoculated mycorrhizal plant respectively. No leaf spot disease in inoculated plant was found. Mycorrhizal dependency (MD) of the vegetables ranged from 15.58% to 31.38%.Among the vegetable studied the "highest mycorrhizal dependency (31.38%) was observed in Brinjal and the lowest MD (15.58%) was observed in Chilli. Okra recorded the second highest MD (26.82%) which was followed by Data (24.79%) and Tomato (18.56 %). N, P, K, Zn and Fe uptake were. influenced significantly by the inoculation of AM fungi over control in all the tested vegetables. A Thesis Submitted to the Faculty of Agriculture, Sher-e-Bangla Agricultural University, Dhaka in Partial fulfillment of the requirements for the degree of MASTER OF SCIENCE (MS) IN PLANT PATHOLOGY 2008-01-01T00:00:00Z http://localhost:8080/handle/123456789/4251 MORPHOLOGICAL AND MOLECULAR CHARACTERIZATION OF BLAST RESISTANT GENOTYPES OF RICE RAHMAN, MD. MOTIUR Genetic divergence analysis was done for blast resistant and susceptible genotypes using 13 morphological characters. The genotypes were grouped into seven clusters according to D2 statistic and Canonical Vector analysis. Plant height, days to flowering, days to maturity, panicle length, number of spikelet/ panicle, number of filled grain/ panicle, number of unfilled grain/ panicle, 1000 filled grain weight(gm), yieldlhill(grn) were indicated as important contribution to genetic divergence in 14 rice genotypes. On the basis of cluster distances, high yielding along with highly susceptible (BRR] dhan 29) genotypes could be crossed highly resistant genotypes (BR 6017-3-3-4-1, ZHONG-YU 7) and BRR] dhan 28 and BRR] dhan 36 could be croosed with QING LIALI NO] for the development of blast resistant rice varieties. A total of 8 microsatellite and 3 minisatellite or VNTR markers were used for studying molecular variabi lity across 14 blast resistant and susceptible rice genotypes. A total of 33 alleles were detected at the loci of 8 microsatellite markers across 14 blast resistant and susceptible rice genotypes. The number of alleles per locus ranged from 2 alleles (RM ) OS) to 5 alleles (RM21, RMSO, RM531), with an average of 4.13 alleles across the 30 loci. The Polymorphism Information Con ten! (PIC) values ranged from 0.2S0 (RMIOS}'to 0.726 (RM21). PJC value revealed that RM21 was considered as the best marker for 14 rice genotypes. The two dimensional graphical view of Principal Coordinate Analysis (PCoA) for blast resisitant genotypes showed the genotypes ZhongYu7, OM1207, SIPI692033, BRRI dhan29, BRRI dhan36, QINGLlALJ 1, JR 60913-42-3-3-2-2 and NJ70507 were found placing far away from the centroid of the cluster and rest of the genotypes were placed more or less around the centroid. The genetic similarity analysis using UPGMA clustering system generated nine genetic clusters with similarity coefficient of 0.66 for the study with blast disease resistance. The pair-wise genetic dissimilarity coefficients indicated that the highest genetic distance was obtained between NJ70507 with BR36 & BR29 for blast disease. In order to develop blast resistant varieties and broaden the genetic base of rice varieties new breeding program should initiated preferably using the parents, BRRldhan29 and NJ70507 and BRRldhan36 and NJ70507 and to create genetic variability among these two modem rice varieties. Both molecular and morphological data did not show the similar results. So, use of more molecular markers might be resolved the results of morphological analysis. A Thesis Submitted to the Faculty of Agriculture, Sher-e-Bangla Agricultural University, Dhaka in Partial fulfillment of the requirements for the degree of MASTER OF SCIENCE (MS) IN PLANT PATHOLOGY 2008-01-01T00:00:00Z http://localhost:8080/handle/123456789/4250 MYCORRHIZAL STATUS OF CROPS GROWN IN ARSENIC AFFECTED AREAS OF MANIKGANJ DISTRICT AND ROLE OF MYCORRHIZAE ON GROWTH OF SELECTED CROS IN ARSENIC AMENDED SOIL SABA, NIBIR KUMAR Crops are often contaminated with arsenic in Bangladesh. This result from irrigation water contaminated with arsenic and leads to problems in human health. Mycorrhizal fungi have their most significant effect on P uptake and have also been shown to reduce arsenic contamination 10 the crops. The present experiment was performed to know the mycorrhizal status of different crops root collected from different arsenic affected villages of Manikganj district. This study also determined the role of arbuscular mycorrhizal fungi on crops growth in arsenic amended soil. Three crops (tomato. radish and garlic) were grown in arsenic amended soils with or without mycorrhizal inoculation. Three levels of arsenic concentrations (1 Oppm, IOOppm and 500ppm) were used. At higher concentration of arsenic, the seed germination was affected more than the other treatments. A positive germination response to AMF inoculation was observed in all the selected crops. In case of garlic, the seed germination, all physical growth was higher at 500ppm treated pots but lower in other two crops. In case of tomato and radish shoot height, root length, number of leaves, both fresh and dry weight of shoots and roots, mycorrhizal root colonization and percent vigor were higher in AMF inoculated pots in comparison to their respective treatments and decreased significantly with the increase rate of arsenic concentrations. Higher nutrient uptake and less arsenic content were recorded in rnycorrhiza inoculated plants. The findings indicate that AMF inoculation not only minimize arsenic toxicity but also can increase growth and nutrient uptake of crops. A Thesis Submitted to the Faculty of Agriculture, Sher-e-Bangla Agricultural University, Dhaka in Partial fulfillment of the requirements for the degree of MASTER OF SCIENCE (MS) IN PLANT PATHOLOGY 2008-01-01T00:00:00Z http://localhost:8080/handle/123456789/4249 MYCORRHIZAL STATUS OF CROPS GROWN IN ARSENIC AFFECTED AREAS OF SONARGAON AND INFLUENCE OF MYCORRHIZAE ON GROWTH OF SELECTED CROPS IN ARSENIC AMENDED SOIL AKHTER, BONYA The contamination of crops because of irrigation with arsenic contaminated water causing problem in human health in Bangladesh. Mycorrhizal fungi can reduce the contamination of arsenic toxicity to the crops. This study were conducted the mycorrhizal status of different crop roots collected from different arsenic (As) affected villages of Sonargaon area and also examined the role of arbuscular mycorrhizal fungi on some selected crops (wheat, spinach and red amaranthus) grown _inarsenic amended soil. Plants were grown with or without arbuscular mycorrhizal fungi (AMF) inoculation in soil amended with three levels of arsenic solution (1Oppm.1OOppmand 500ppm). The higher concentration of arsenic contaminated soil affected the seed germination. At 500ppm, treated soil completely inhibited seed germination of red amaranthus but little germination was found in wheat and spinach.' A positive germination response to AMF was observed in all the selected crops. Root length, shoot height, leaf number, fresh and dry weight of shoot and root, mycorrhizal root colonization, per cent vigority, nutrient (N, P, K and S) uptake increased significantly due to mycorrhizal infection and decreased significantly with increasing arsenic concentrations. Mycorrhizal inoculation reduced As concentration in shoots of red amaranthus. Thus, crops with compatible AMF inoculation can minimize arsenic toxicity and increase plants growth and nutrient uptake. A Thesis Submitted to the Faculty of Agriculture, Sher-e-Bangla Agricultural University, Dhaka in Partial fulfillment of the requirements for the degree of MASTER OF SCIENCE (MS) IN PLANT PATHOLOGY 2008-01-01T00:00:00Z