Year 2021

EFFECTS OF FEEDING MINT LEAF (Mentha pulegium L.) AS AN ALTERNATIVE TO ANTIBIOTICS ON PERFORMANCE OF BROILER

2023-08-28T04:19:19Z

EFFECTS OF FEEDING MINT LEAF (Mentha pulegium L.) AS AN ALTERNATIVE TO ANTIBIOTICS ON PERFORMANCE OF BROILER ISLAM, KHNDAKAR REZAUL The research was conducted to determine the effects of feeding mint leaf powder on the performance of broiler chicken. A total of 225 day-old Lohman meat broiler chicks were allocated randomly to five treatment groups with three replications having 15 birds per replication. There were five groups. e. g. T 0 (Basal feed), T 1 (Basal feed+Antibiotic), T 2 (Basal feed+1.0% mint leaf powder), T 3 (Basal feed+1.50% mint leaf powder) and T 4 (Basal feed+2.0% mint leaf powder). Result demonstrated that the body weight (g) was insignificantly (P>0.05) higher in T 2 (1965) and lowest in T 1 (1838.66). The body weight gain (g) was insignificantly (P>0.05) higher in T 2 (1923) than other groups including control group . Feed consumption (g) was significantly (P<0.05) higher in T 3 (2489) than other groups including control group. The better feed conversion ratio (FCR) was significantly (P<0.05) observed in T 2 (1.20) group compared to other groups. Dressing percentage was insignificantly (P<0.05) higher in T 3 (70.25) and lower in T 0 (67.95). The weight (g) of liver, heart, spleen and gizzard in T 2 was significantly higher (P<0.05). The weight (g) of thigh, drumstick and back in T 2 were insignificantly (P>0.05) higher. Insignificantly (P>0.05) survivability rate (%) was better in all groups except T 4 . BCR is insignificantly higher (P>0.05) in treatment group T 2 (1.91) than control group (T 0 ). Average counts of E. coli and Salmonella sp. bacteria were insignificantly (P>0.05) lowest in T 3 (6.18) and T 4 (4.56) respectively. Significantly (P<0.05) more WBC was found in T 0 (14.60) and less in T 4 (1.20). But, T 2 (12.23) group was better result than others group. Significantly (P<0.05) the highest lymphocyte was found in T 4 (3.23) and lowest in T 3 (2.220). But, T 2 (2.68) treatment group has better result than other groups including control group. Significantly (P<0.05) the highest granulocyte was found in T 0 (11.73) and lowest in T 1 (3.55). But, T 2 (9.69) treatment group has better result than others group. Better result was found in T 2 (basal feed+1.0% mint leaf powder) group than others including control group (T 0 ). A Thesis Submitted to the Faculty of Animal Science & Veterinary Medicine, Sher-e-Bangla Agricultural University, Dhaka-1207, in Partial Fulfillment of the Requirements for the Degree of MASTER OF SCIENCE (MS) IN ANIMAL NUTRITION

SUPPLEMENTATION OF BOVINE SERUM ALBUMIN AND OVURELIN HORMONE TO DETERMINE AN EFFECTIVE MEDIA ON IN VITRO MATURATION AND FERTILIZATION OF CATTLE OOCYTES

2022-11-14T10:54:45Z

SUPPLEMENTATION OF BOVINE SERUM ALBUMIN AND OVURELIN HORMONE TO DETERMINE AN EFFECTIVE MEDIA ON IN VITRO MATURATION AND FERTILIZATION OF CATTLE OOCYTES Akhter, Nasrin The present experiment has been undertaken to know the effect of hormone and protein supplementation on in vitro maturation and fertilization of cattle oocytes. Ovaries from slaughter were brought to the laboratory and washed in a saline solution at 30℃ for 2 to 3 times. Cumulus oocytes complexes (COCs) were collected by aspiration method and 475 follicles were aspirated out of 631 visible follicles on the surface of both ovaries from CL group (Luteal phase) and without CL (Follicular phase),Where the COCs were classified as normal (Grade A and Grade B) and abnormal (Grade C and Grade D) groups, the highest numbers of normal COCs were found in the ovary without CL (3.11±0.21%). The COCs were matured for 48 hours in TCM-199 basic medium supplemented 10% Bovine Serum Albumin (BSA) and Gonadotropin Hormone (Ovurelin). Cumulus Cell expansion of COCs were studied in TCM-199 cultured media with Ovurelin and 10% BSA. COCs were fertilized with frozen semen after maturation in TCM-199 supplemented with 10% BSA and 10.15µg/ml Ovurelin. The maturation rate is significantly higher at (66.25±2.67%) when 10% BSA and 10.15µg/ml Ovurelin used combined. Similarly, the fertilization rate was significantly higher (P<0.05) and high number of normal pronuclei formation (2PN) (66.25±2.67%) was found when supplemented with 10% BSA and 10.15µg/ml Ovurelin. Thus, it can be concluded that the combined media of 10% BSA and Ovurelin might be used as a good supplement for in vitro maturation and fertilization of this study. A THESIS Submitted to the Department of Animal Nutrition, Genetics and Breeding, Sher-e-Bangla Agricultural University, Dhaka-1207 in partial fulfillment of the requirements for the degree of MASTER OF SCIENCE (MS) IN ANIMAL BREEDING AND GENETICS

DETERMINATION OF EFFECTIVE MEDIA AND ITS HORMONE SUPPLEMENTATION ON IN VITRO MATURATION AND FERTILIZATION OF COW OOCYTES

2022-11-14T10:52:22Z

DETERMINATION OF EFFECTIVE MEDIA AND ITS HORMONE SUPPLEMENTATION ON IN VITRO MATURATION AND FERTILIZATION OF COW OOCYTES ROY, SOMA RANI The present experiment was conducted to determine an effective basic medium and its hormone supplementation for in vitro maturation and fertilization of cow oocytes. To determine an effective basic medium and hormone the effect of Tissue Culture Media 199 (TCM 199), Tissue Culture Media 199 (TCM 199) supplemented with different doses of Gonadotropin (0.5, 1,5,10 and 15 µg/ml) hormone on in vitro maturation and fertilization (of cow oocyte were evaluated throughout the study. Synthetic ovurulin used as substitute of gonadotropin. Cumulus Oocytes Complexes (COCs) were collected from cow ovaries by aspiration method and matured for 48 hours at 37.5ºC with 5% CO 2 in humidified air in basic and supplemented media. Significantly higher (p<0.05) number of follicles were aspirated in ovaries without CL (Corpus Luteum) (4.59±0.27) compared with ovaries containing CL (3.05). Consequently higher numbers of COCs were found in ovaries without CL (4.61) than ovaries with CL (1.98). The maturation rate was significantly higher (65.71) in (TCM 199 with Gonadotropin10 µg/ml) medium than those of other treatment. Higher maturation rate was found up to the 10 µg/ml Gonadotropin supplementation but when the dose exceeds 10 µg/ml the maturation rate began to decline. In vitro fertilization rate depends on, the condition of the used semen whether it is fresh or frozen, oocyte quality and maturation rate. Comparatively higher fertilization rate based on pronucli formation was found in Tissue Culture Media 199 (TCM 199), supplemented with Gonadotropin 10 µg/ml (40.14) than other media. Therefore, ovaries without CL could be used in Tissue Culture Media 199 (TCM 199), basic medium supplemented with Gonadotropin (10 µg/ml) level may be used as a basic medium for optimum in vitro maturation rate of cow’s oocytes in Bangladesh. A Thesis Submitted to the department of Animal Nutrition, Genetics and Breeding Sher-e-Bangla Agricultural University, Dhaka in partial fulfillment of the requirements for the degree of MASTER OF SCIENCE (MS) IN ANIMAL BREEDING AND GENETICS

EVALUATION OF REPRODUCTIVE PERFORMANCE OF INDIGENOUS BUFFALO AT SREEMANGAL UPAZILA

2022-08-01T06:26:24Z

EVALUATION OF REPRODUCTIVE PERFORMANCE OF INDIGENOUS BUFFALO AT SREEMANGAL UPAZILA HIMEL, ALAUDDIN The reproductive performance of indigenous buffaloes at Sreemangal Upazila under Moulovibazar district was studied. Thirty (30) families were interviewed by written interviewed paper from Sreemangal Upazilla under Moulovibazar district in 2021. Out of 30 families 26 own 33 milch buffaloes, 17 dry buffaloes, 12 heifer calves, 7 bull calves and 10 bullocks which were counted for calculation. This study demonstrated that the distribution of buffalo populations were scattered and the average buffalo populations were 3.038 per house-holds, age at puberty 44.06 ± 2.13 months, lactation length 204.70±20.68 days, milk yield 1.88±0.702 liters/day, gestation period 307.00±2.11 days, body weight 27.26±1.55 kg/calf, age at first calving 53.84±1.48 months, calving interval 1.72±.548 years and average post partum heat period was 147.6±18.68 days. These data will be helpful for future research and also give a guideline for buffalo development in Bangladesh. A Thesis Submitted to the Faculty of Animal Science & Veterinary Medicine, Sher-e-Bangla Agricultural University, Dhaka1207, in partial fulfillment of the requirements for the degree of MASTER OF SCIENCE (MS) IN ANIMAL BREEDING AND GENETICS SEMESTER: June-December, 2021