http://localhost:8080/handle/123456789/3614 2026-04-18T21:08:21Z 2026-04-18T21:08:21Z ISLAM, SUMAIYA http://localhost:8080/handle/123456789/4714 2022-12-20T10:46:47Z 2021-12-01T00:00:00Z

POPULATION DYNAMICS AND MORPHOLOGICAL IDENTIFICATION OF LICE IN COMMERCIAL LAYER CHICKEN ISLAM, SUMAIYA Poultry is a promising sector in Bangladesh that is growing at a rapid pace and has grown to become the most important and advanced segment of the livestock sector in the country. Commercial poultry is infected with a variety of parasites. As a result, the goal of this research was to look into the population dynamics and morphological identification of lice in commercial poultry farms of Kishoreganj, Gazipur, Mymensingh, Tangail and Narsingdi. From November 2020 to April 2021, a total of 245 layer chickens were examined for lice infestation. All the lice were identified microscopically at the Laboratory of Microbiology and Parasitology. A total of 149 chickens (60.81 %) were infested with lice out of 245 evaluated. During the study, four lice species were identified: Menacanthus stramineus, Menopon gallinae from sub-order Amblycera and Gnoiocotes gallinae, Lipeurus caponis from sub-order Ischinocera. Among them, M. stramineus was the most prevalent species (60.78%) detected in breast and thigh, followed by M. gallinae (49.02%) in body feathers, L. caponis (34.90%) in wing feather and G. gallinae (15.03%) in fluff. The highest prevalence of lice was found in Gazipur (83.93%) followed by Mymensingh (80.65%), Tangail (72.73%), Narsingdi (66.67%). No lice were detected in Kishoreganj. This research strongly suggests that poultry lice are a major problem in commercial poultry chicken. It is indeed necessary to develop an effective control measure to improve output of commercial poultry in Bangladesh. A Thesis Submitted to Department of Microbiology and Parasitology Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka-1207 Requirements for the degree of MASTER OF SCIENCE (MS) IN PARASITOLOGY Semester: July-December/2021

2021-12-01T00:00:00Z MAHMOOD, MD. SAIFULLAH http://localhost:8080/handle/123456789/4713 2022-12-20T04:17:58Z 2021-01-01T00:00:00Z

ISOLATION, IDENTIFICATION AND ANTIBIOTIC SENSITIVITY OF ESCHERICHIA COLI AND SALMONELLA SPECIES FROM HOSPITAL WASTE WATER IN DHAKA CITY MAHMOOD, MD. SAIFULLAH The use of antibiotics in the hospitals for patient care, and disinfection is a global scenario, but it has become a threatening issue for all. Antibiotics are partially metabolized and residual quantities reach into hospital waste water. A cross-sectional study was conducted between January and June 2021 from different hospitals waste water to identify the Escherichia coli (E. coli) and Salmonella spp. A total of 100 samples were collected for bacteriological analysis. Bacteria were identified using morphological, cultural and biochemical characterization. The E. coli showed metallic sheen and pink colored colonies on EMB agar and MacConkey agar respectively. It was gram negative and showed small rod shaped arranged in single or pair shaped. It was noticed as positive to Indole, MR and catalase test but negative to VP test. In case of Salmonella spp, it was observed as black smooth colonies on SS agar. These bacteria were observed as short rod-shaped gram-negative bacteria. The MR and catalase test were positive but Indole and VP test were negative. The occurrence of E. coli and Salmonella spp. were 80% and 87%, respectively. The antibiotic sensitivity test indicated that the both types of isolated E. coli and Salmonella spp. were highly resistant to tetracycline (100%). Besides, E. coli was resistant to ampicillin (93.75%) and sensitive to ciprofloxacin (65%), streptomycin (62.5%) and gentamycin (93.5%) but Salmonella spp. was highly resistant to ampicillin (100%) and sensitive to ciprofloxacin (73.86%), gentamycin (79.54%) and streptomycin (34%). Hospital waste water contained antibiotic resistant bacteria that was reported as alarming voice to public health. A Thesis Submitted to the Faculty of Animal Science and Veterinary Medicine Sher-e-Bangla Agricultural University for the Partial Fulfillment of the Requirements for the Degree of MASTER OF SCIENCE (MS) IN MICROBIOLOGY

2021-01-01T00:00:00Z RAHMAN, MD. MASHIUR http://localhost:8080/handle/123456789/4712 2022-12-20T04:15:50Z 2021-01-01T00:00:00Z

IDENTIFICATION AND ANTIBIOGRAM PROFILING OF ESCHERICHIA COLI AND SALMONELLA SPP. ISOLATED FROM SUPPLIED WATER OF SELECTED CATTLE FARMS IN DHAKA RAHMAN, MD. MASHIUR The present study was carried out to isolate, identify and to know antibiogram profiling of Escherichia coli and Salmonella spp. from the supplied drinking water in different cattle farms in Dhaka city within the period of October, 2020 to April, 2021. A total of 100 fresh water samples were randomly collected from different cattle farms and transported to the Microbiology and Parasitology laboratory of Sher-e-Bangla Agricultural University for microbiological analysis. Primary culture was done in nutrient broth and nutrient agar. Pure culture was obtained from different selective media. Bacteria were identified by observing the growth properties in different media, staining properties and biochemical tests. The characteristics of E. coli colonies were red to bright pink colored in MacConkey agar and greenish red colored with faint metallic sheen in EMB (Eosin Methylene Blue) agar. The characteristics of Salmonella spp. colonies were black colored in SS agar and pink colored in MacConkey agar. In Gram’s staining, E. coli revealed gram-negative, pink color, small rod-shaped appearance, arranged in single or paired short, and Salmonella spp. revealed gram negative, short rod shaped, singly arranged. The occurrence of E. coli was 46% and Salmonella spp. was 37%. Pure isolates were subjected to antibiogram by disc diffusion method against 5 different antibiotics including streptomycin (Str), ampicillin (Amp), ciprofloxacin (Cip), gentamycin (Gen) and tetracycline (Te). Highest number of E. coli isolates showed resistance to gentamycin (43%) followed by ampicillin (38%) and tetracycline (26%). In case of Salmonella spp., highest number of E. coli isolates showed resistance to gentamycin (31%) followed by ampicillin (31%) and tetracycline (27%). This study revealed that water samples collected from different cattle farms of Dhaka city were contaminated with multiple species of multidrug resistant bacteria which may pose risk for both animal and human health. A Thesis Submitted to the Faculty of Animal Science and Veterinary Medicine Sher-e-Bangla Agricultural University, Dhaka-1207, In Partial Fulfillment of the Requirements for the Degree of MASTER OF SCIENCE (MS) IN MICROBIOLOGY

2021-01-01T00:00:00Z HASNAIN, MD. NEHAL http://localhost:8080/handle/123456789/4711 2022-12-20T04:13:43Z 2021-01-01T00:00:00Z

OCCURRENCE AND ANTIBIOTIC SENSITIVITY PROFILING OF STAPHYLOCOCCUS AUREUS ISOLATED FROM RAW CHEVON HASNAIN, MD. NEHAL This study aimed to determine the prevalence as well as the antibiotic sensitivity of Staphylococcus aureus in market meat (chevon) sold at the different wet markets in Dhaka city. A total of 120 samples of raw chevon were collected from the selected area. S. aureus was isolated from the samples using standard microbiological methods with their Total Viable Count (TVC). The highest TVC found on a sample was log 10 9.22 CFU/gm and the lowest TVC was log 10 9.04 CFU/gm. Cultural characteristics, biochemical testing, and gram staining were used to isolate and identify bacteria. Prevalence rate of S. aureus ware 47.5%. Isolates were investigated for antibiotic sensitivity profiling by using a Kirby-Bauer disc diffusion assay against five commonly used antibiotics used in goats. S. aureus showed the highest sensitivity to gentamycin (57.9%), followed by ciprofloxacin (56.14%), and the highest resistance pattern was shown against amoxicillin (100%), followed by ampicillin (92.98%), and tetracycline (63.15%). According to the findings of this study, chevon contains multidrug-resistant S. aureus pathogens on market meat. The presence of multidrug-resistant S. aureus bacteria in market meat raises serious public health concerns, which can be transmitted to humans through direct contact or the food chain, posing a public health risk. A Thesis Submitted to the Faculty of Animal Science and Veterinary Medicine Sher-e-Bangla Agricultural University, Dhaka-1207 In Partial Fulfilment of the Requirements for the degree of MASTER OF SCIENCE (MS) IN MICROBIOLOGY

2021-01-01T00:00:00Z