http://localhost:8080/handle/123456789/3201 2026-04-18T14:14:49Z 2026-04-18T14:14:49Z KHATUN, MST. REHENA http://localhost:8080/handle/123456789/3215 2020-03-12T08:12:13Z 2019-12-01T00:00:00Z

SURVEY ON WHEAT BLAST AND MORPHOLOGICAL CHARACTERIZATION AND IN-VITRO MANAGEMENT OF MAGNAPORTHE ORYZAE TRITICUM THROUGH BOTANICALS KHATUN, MST. REHENA Wheat blast disease caused by Magnaporthe oryzae triticum (MoT) has become a serious constrain in increasing the wheat area of infection and decreasing the cultivable area of the crops. A survey was conducted in 30 villages of blast infected South-Western wheat growing region of Bangladesh during January to April, 2019. During the survey at Meherpur Sadar, Mujibnagar and Chuadanga Sadar Upazilla, the highest incidence and severity was recorded in cultivar BARI Gom-24 (Pradip) at Kutubpur, Monkhali and Parkrisnapur village and the lowest incidence and severity was found in BARI Gom-26 and BARI Gom-28 at Pirojpur, Charulia and Bollolpur village. Thirty-five MoT isolates were isolated, identified and tested their pathogenicity. Growth response and cultural characteristics of the MoT isolates were done on PDA. In the present study, the highest radial mycelial growth observed on the isolate CHMoT 09 (25.67 mm) on the 7 days, CHMoT 06 (56.33 mm), CHMoT 07 (55.83 mm), CHMoT 08 (56.33 mm) and CHMoT 09 (57.00 mm) on the 14 th days incubation and CHMoT 08 (75.50 mm) on the 30 days incubation. Based on mycelial growth per day, isolates of M. oryzae triticum were classified into three cluster groups, cluster I, cluster II, cluster III that indicates the presence of a morphologically diversified group of pathogens. Aloe vera (Allovera leaf) extracts and Nigella sativa (Black cumin seeds) extracts @ (1:1 w/v) concentration were found the most effective botanicals to reduce mycelial growth of M. oryzae triticum under in-vitro condition. However, this experiment with more plant extracts needs to be coined out to assess the field efficacy of these botanical extracts with different concentrations and frequencies in controlling blast disease of wheat. th th A Thesis Submitted to the Faculty of Agriculture Sher-e-Bangla Agricultural University, Dhaka in partial fulfilment of the requirements for the degree of MASTER OF SCIENCE IN PLANT PATHOLOGY

2019-12-01T00:00:00Z ISLAM, MD. ZAHIDUL http://localhost:8080/handle/123456789/3214 2020-03-12T08:08:34Z 2019-12-01T00:00:00Z

SURVEILLANCE AND MANAGEMENT OF WHITE RUST (Albugo candida) DISEASE OF RED AMARANTH FOR SEED PRODUCTION ISLAM, MD. ZAHIDUL Experiments were conducted in Sher-e-Bangla Agricultural University, Dhaka-1207, to identify the causal organism of white rust disease of red amaranth (Amaranthus tricolor L.) and for its suitable management for seed production during rabi and kharif seasons of 2018-19. Eight treatments including chemicals, botanicals, bio-pesticides viz. Ridomil gold 68WG (Mencozeb + Metalaxyl @ 0.2%), Autostin 50 WP (Carbendazim @ 0.2%), Dithane M 45 (Mancozeb @ 0.2%), Goldton 50WP (Copper oxychloride @ 0.2%), Bordeaus mixture (CaO + CuSO @ 1%), G-Derma ( Trichoderma sp.) @ 0.3%, Garlic bulb extract 1:1 (w/v) (Allium sativum @ 2%), and Allamanda leaf extract 1:1 (w/v) (Alamanda cathertica @ 2%) were considered for the management of white rust disease of Red amaranth (Amaranthus tricolor L.). Four foliar sprays were done at seven days interval, started from 7 days after disease appeared. The field experiments were conducted by following RCBD design with three replications. Obligate fungi Albugo candida was successfully identified by microscopic study as the causal organism of white rust disease of red amaranth. Among the treatments, Ridomil gold 68WG gave best result against white rust disease of red amaranth. Moreover, Allamanda leaf extract showed better effect and Autostin 50WP had moderate effect against the disease compare to other treatments. Growth and seed yield of red amaranth varied significantly among the treatments. In Rabi season of 2018, After 4 4 spray, the lowest plant incidence was recorded in Ridomil gold (24%) followed by Allamanda leaf extract (31%). Similarly, the lowest disease severity was found in Ridomil gold (1.83%) followed by Allamanda leaf extract (2.43%). Ridomil Gold reduced 63.07% plant incidence, 62.78% leaf incidence and 84.31% disease severity at 61 DAS over control. However, Allamanda leaf extract reduced 52.30% plant incidence, 52.78% leaf incidence and 79.17% disease severity over control. Similar results also found in kharif season of 2019 where after 4 th spray, the lowest plant incidence was recorded in Ridomil Gold (18.66%) which was statistically identical with Allamanda leaf extract (22%) and the lowest disease severity was found in Ridomil gold (1.93%) followed by Allamanda leaf extract (2.23%). At 57 DAS, Ridomil Gold reduced plant incidence (69.73%), leaf incidence (65.71%) and disease severity (88.41%) over control. However, Allamanda leaf extract reduced plant incidence (64.32%), leaf incidence (60.48%) and disease severity (86.61%) over control. Considering the overall performance of the treatments, Ridomil Gold 68WG showed the best effect in controlling white rust disease. Thus, Ridomil Gold 68WG could be used for seed production of red amaranth. Moreover, Allamnada leaf extract could be used as an ecofriendly approach for white rust disease management to ensure safe crop production. th A Thesis Submitted to the Faculty of Agriculture Sher-e-Bangla Agricultural University, Dhaka in partial fulfilment of the requirements for the degree of MASTER OF SCIENCE IN PLANT PATHOLOGY

2019-12-01T00:00:00Z AKHTER, AFSANA http://localhost:8080/handle/123456789/3213 2020-03-12T07:59:05Z 2019-12-01T00:00:00Z

GROWTH AND SPORULATION OF Magnaporthe oryzae CAUSING BLAST OF RICE IN SELECTED CULTURE MEDIA AND ITS In vitro MANAGEMENT AKHTER, AFSANA An experiment was carried out at Molecular Biology and Plant Virology Laboratory, under the Department of Plant Pathology, Sher-e-Bangla Agricultural University, Dhaka-1207. The study was designed to aid in further experiments and find out the suitable measures to control rice blast pathogen. The study consisted of the isolation of rice blast pathogen from the field sample for morphological study, the evaluation of 12 different selected solid media to have a comparative analysis of their performance on morphological study of Magnaporthe oryzae, the pathogenicity test through Koch’s postulates and evaluation of efficacy of four latest chemicals against Magnaporthe oryzae. Diseased samples were collected randomly from the severely rice blast infected field from Mymensingh and Thakurgaon districts. The disease affected plant parts were collected and conveyed to the laboratory for in-vitro experiments. The morphological study was done by observing two isolates from the collected samples. It is evident that the disease was caused by Magnaporthe oryzae as the isolates produced pyriform, 3 celled hyaline conidia which had two septa. Among the 12 selected media PDA, CDA and PSA gave better performance in terms of mycelial growth and PSA, PR S DA for sporulation. On the other hand, PA (Prune agar) and SA (Starch agar) also gave good performance and these media can be a good alternative for morphological study and isolation purpose. Among the four selected fungicides, Amister top 325 SC and Blastin 75 WDG gave 100% mycelial growth inhibition with 500 ppm, 1000 ppm and 1500 ppm doses against Magnaporthe oryzae invitro. Besides, Acibean 28 SC and Deconil 500 SC had lower effect on rice blast pathogen over control. A Thesis Submitted to the Faculty of Agriculture Sher-e-Bangla Agricultural University, Dhaka in partial fulfilment of the requirements for the degree of MASTER OF SCIENCE IN PLANT PATHOLOGY

2019-12-01T00:00:00Z ASHRAFI, MST. LAILA http://localhost:8080/handle/123456789/3209 2020-03-12T05:05:52Z 2019-12-01T00:00:00Z

ASSESSMENT OF WHEAT BLAST INTENSITY IN SOUTH WEST OF BANGLADESH AND CHARACTERIZATION OF MAGNAPORTHE ORYZAE TRITICUM ON DIFFERENT CULTURE MEDIA ASHRAFI, MST. LAILA This study was carried out to determine the incidence and severity of wheat blast, isolation and pathogenicity of Magnaporthe oryzae triticum (MoT) isolates and evaluation on different culture media for their growth and cultural characteristics. Wheat blast were assessed in 30 farmers' fields in two districts during the Rabi cropping season in February to March 2019, in major wheat growing areas viz. Meherpur, Mujibnagar and Chuadanga. The results of the assessment revealed that the wheat blast incidence and severity varied from low to high depending on the survey sites and crop varieties. The highest incidence and severity, 100% for all areas were recorded in BARI Gom-24 (Prodip) followed by BARI Gom 26 and BARI Gom 28 in entire three districts. A total of 15 MoT isolates were identified and characterized based on their growth parameters. Among them, ten isolates such as ME MoT13, ME MoT14, ME MoT15, ME MoT16, MU MoT13, MU MoT14, MU MoT15, MU MoT16, CH MoT11 and CH MoT12 were evaluated on the eleven culture media such as Oat meal agar, Corn meal agar, Prune agar, Yeast extract agar, V5 agar, Wheat straw agar, Potato dextrose agar, Potato carrot agar, Wheat seed extract agar, Wheat flour agar and Potato sucrose agar. MoT isolates showed comparatively higher mean radial mycelial growth on OMA (51mm) followed by Yeast extract agar (48.33mm) and Wheat flour agar (44.57mm) at 4 DAI. Minimum mean radial mycelial growth was recorded on CMA (16.2mm). The radial mean mycelial growth was also sufficient on PDA, PSA and Wheat straw agar. Key words: Wheat blast, incidence, severity, culture media, Magnaporthe oryzae triticum A Thesis Submitted to the Faculty of Agriculture Sher-e-Bangla Agricultural University, Dhaka in partial fulfilment of the requirements for the degree of MASTER OF SCIENCE IN PLANT PATHOLOGY

2019-12-01T00:00:00Z