Year 2017http://archive.saulibrary.edu.bd:8080/xmlui/handle/123456789/28192024-03-28T19:14:17Z2024-03-28T19:14:17ZMOLECULAR DIVERSITY ANALYSIS AND POLYMORPHISM STUDY IN COTTON (Gossypium hirsutam L.) GENOTYPES THROUGH RAPD MARKERSWAHAB, MD. ABDULhttp://archive.saulibrary.edu.bd:8080/xmlui/handle/123456789/22862019-10-17T11:16:53Z2017-01-01T00:00:00ZMOLECULAR DIVERSITY ANALYSIS AND POLYMORPHISM STUDY IN COTTON (Gossypium hirsutam L.) GENOTYPES THROUGH RAPD MARKERS
WAHAB, MD. ABDUL
The experiment was conducted at the Laboratory of the Department of Biotechnology, Sher-eBangla
Agricultural
University
(SAU),
Dhaka-
1207,
Bangladesh.
The
period
of
the
experiment
was
March 2018 to November 2018. Cotton (Gossypium hirsutum L.) is the leading fiber crop
and the second most important oil seed crop in the world. This study aimed to analyze the
molecular diversity among 9 cotton genotypes collected from Cotton Development Board,
Bangladesh using the Random Amplified Polymorphic DNA primer. A total of 11 RAPD
primers were used to determine polymorphism among the genotypes and finally 6 of the primers
produced scorable DNA bands. Some total of with 30 DNA bands were obtained and among
them 9 were polymorphic bands. The ranged of DNA amplification varied from 160 to 1500 bp.
The rate of polymorphism was calculated about 31.57%. Genetic diversity ranged from 0.22 to
0.44 with an average value of 0.30. Nei’s genetic distance ranging from 0.1667 to 0.6667 and
most importantly PIC value ranged from 0.18 to 0.35 with an average value of 0.27. The PIC
value indicates that most of the studied cotton genotypes was moderately diversified and
homogenous as well as no heterozygosity found. A dendogram indicating the relative genetic
similarity of the Bangladeshi cotton genotypes was constructed which followed in two major
cluster (A and B) among the studied material. The results also showed that the genotypes can be
separated from each other at the molecular level by taking advantage of some of the RAPD
markers. The Cotton Development Board, Bangladesh committed to work on cotton
improvement and so for this was the first time in CDB to investigate molecular diversity and
DNA profiling in cotton using CDB collected cotton genotypes. This experiment can be used as
a baseline for the future molecular research work on cotton genotypes in Bangladesh.
DEPARTMENT OF BIOTECHNOLOGY
SHER-E-BANGLA AGRICULTURAL UNIVERSITY
DHAKA-1207
2017-01-01T00:00:00ZDNA MARKER BASED HYBRIDITY TEST OF DIFFERENT EGGPLANT (Solanum melongena L.) HYBRIDSHOSSAIN, MAHBUBAhttp://archive.saulibrary.edu.bd:8080/xmlui/handle/123456789/22852019-10-17T11:17:04Z2017-01-01T00:00:00ZDNA MARKER BASED HYBRIDITY TEST OF DIFFERENT EGGPLANT (Solanum melongena L.) HYBRIDS
HOSSAIN, MAHBUBA
An experiment was carried out to identify the hybrid variety of eggplant through SSR marker.
Four eggplant hybrids vizMEH5003, MEH5006, MEH5009, MEH5012 and their corresponding
parents were used as experimental materials. The hybrid varieties of eggplant were developed by
Metal Seed Ltd., Dhaka, Bangladesh. The experiment was conducted at the Department of
Biotechnology, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh. Initially different
SSR primerswere used to establish polymorphism among the parents of each hybrid. The
identified polymorphic primers were used to screen the hybrid variety together with
corresponding parents. It was noticed that only one primer viz MEH SSR-04 showed
polymorphism in the parents of MEH5009 hybrid. This primer was used for the detection of
hybrid variety. A 150bp DNA fragment was amplified in male parent and hybrid variety which
was absent in female genotype. This DNA band can be used for large scale screening of that
specific hybrid variety for seed purity in commercial purpose. The parents of other hybrid
showed monomorphic DNA band and some of the SSR primerswere not amplified in any
eggplant genotype under investigation. The confirmation of genetic purity in seeds is very
important for identification of genetic contamination and hybrid purity for farmers and plant
breeders before sale of the seed to public domain. The genetic purity test by conventional
method called Grow Out Test
DEPARTMENT OF BIOTECHNOLOGY
SHER-E-BANGLA AGRICULTURAL UNIVERSITY
DHAKA-1207
2017-01-01T00:00:00ZSTUDY ON POTATO (Solanum tuberosum L.) REGENERATION CAPABILITY IN AMMONIUM NITRATE FREE MEDIUM COMPOSITIONHENA, HASNAhttp://archive.saulibrary.edu.bd:8080/xmlui/handle/123456789/20402019-10-17T11:17:15Z2017-01-01T00:00:00ZSTUDY ON POTATO (Solanum tuberosum L.) REGENERATION CAPABILITY IN AMMONIUM NITRATE FREE MEDIUM COMPOSITION
HENA, HASNA
The experiment was conducted at the Biotechnology Laboratory, Department of Biotechnology,
Sher-e-Bangla Agricultural University, Dhaka, Bangladesh during the period of January, 2016 to
June, 2017 to evaluate the performance of ammonium nitrate free medium for in vitro
regeneration of a potato (Solanum tuberosum L.) variety, Asterix. Sprouts and nodal segments
were used as explants. A new chemical which is indicated as β chemical had been used in this
research. Five different compositions of the stock solution-01 of the MS medium were used
which were marked as Treatment-01 (standard MS dose, 1962), Treatment-02 (NH
4
free
stock solution), Treatment-03 (NH
4
NO
3
free stock solution with double dose of other
ingredients), Treatment-04 (stock solution-01, developed in the Department of Biotechnology,
SAU, Dhaka ) and Treatment-05 (readymade MS powder). In vitro regeneration of a potato was
successfully achieved in those media. The parameters like shoot length, shoot number, shoot
diameter, node number per plantlet, leaf number per plantlet were highest in Treatment-04 at
14, 21 and 28 days of regeneration. But in case of root number and root length (cm) per
plantlet, Treatment-05 showed the highest result and in some cases it was statistically similar
with Treatment-04. Treatment-02 showed lowest result in each of the said parameter. It might be
due to the low concentration of nitrogen and it indicated that NH
4
x
NO
3
is essential for in vitro
regeneration of potato. Treatment-03 performed moderately in most of the parameters. Asterix
variety showed significant variation in case of Treatment-01
and Treatment-05
on most of the
parameters under studied. Therefore, a relliable medium composition for in vitro potato
regeneration has been established. So, the stock solution-01 which was developed from the
Department of Biotechnology, SAU, Dhaka can be the best alternative to NH
4
in tissue
culture study of plants.
A Thesis
Submitted to the Faculty of Agriculture,
Sher-e-Bangla Agricultural University, Dhaka,
in partial fulfillment of the requirements
for the degree of
MASTER OF SCIENCE (MS)
IN
BIOTECHNOLOGY
2017-01-01T00:00:00ZIN VITRO REGENERATION OF ONION (Allium cepa L.) UNDER SALT STRESS CONDITIONPLABON, MD. ASFIQUR RAHMANhttp://archive.saulibrary.edu.bd:8080/xmlui/handle/123456789/20392019-10-17T11:17:28Z2017-01-01T00:00:00ZIN VITRO REGENERATION OF ONION (Allium cepa L.) UNDER SALT STRESS CONDITION
PLABON, MD. ASFIQUR RAHMAN
The present research was carried out in Biotechnology Laboratory of the
Department of Biotechnology, Sher-e-Bangla Agricultural University, Sher-eBangla
Nagar,
Dhaka-1207
from
the
period
of
September
2016
to
July
2017
for
in
vitro regeneration of onion (Allium cepa L.) under salt stress condition.
Three genotypes namely Faridpuri, Taherpuri and Pusa red (Indian) were used
as investigated genotypes in this study. Shoot tip segments of these genotypes
were cultured in MS (Murashige and skoog, 1962) media having 0.0 (control),
25, 50, 75 and 100 mM NaCl. The shoot tips of experimental onion genotypes
were not significantly affected upto 75 mM salinity level in comparison with
control treatment. The experiment was conducted at two factorial (variety and
Treatment) Completely Randomized Design (CRD) with 3 replications for each
treatment. Salinity response on days required for shoot regeneration varied
significantly from different salinity condition. The genotype Faridpuri was
found maximum tolerant upto 100mM salinity level with 10.60 cm shoot length
and 1.43 cm root length having the highest relative shoot and root growth.
Besides, Pusa red was found as salinity sensitive genotype showing the lowest
7.03 cm shoot length and 0.60 cm root length at 100 mM NaCl treatment.
However, Taherpuri was found tolerant up to 75 mM salinity level with 8.30
cm shoot length and 1.43 cm root length, respectively. The genotypes
Faridpuri, Taherpuri and Pusa red (Indian) can be used for further investigation
in field condition to evaluate their performance at various salinity levels.
Therefore, a convenient in vitro regeneration protocol of onion genotypes
under different salinity level has been developed which can be used for
screening salinity tolerant variety of onion within a very short period of time.
A Thesis
Submitted to the Faculty of Agriculture,
Sher-e-Bangla Agricultural University, Dhaka,
in partial fulfillment of the requirements
for the degree of
MASTER OF SCIENCE
IN
BIOTECHNOLOGY
2017-01-01T00:00:00Z