Abstract:
The study investigated watermelon diseases characterized by contrasting climatic conditions
in the Sylhet and Natore Districts of Bangladesh. Sylhet experiences lower temperatures
and high rainfall, while Natore has higher temperatures and low rainfall. In these
survey regions, 40 watermelon fields were selected, and 10 diseases were observed,
including 4 fungal, 3 bacterial, 2 water mold, and 1 viral disease. The observed diseases
were Anthracnose, Cercospora leaf spot, Fusarium wilt, Gummy stem blight, Downy mildew,
Phytophthora fruit rot, Bacterial fruit blotch, Angular leaf spot, Yellow vine, and Watermelon
mosaic disease. Molecular analysis was done in the Plant Pathology Lab at Sher-eBangla
Agricultural University using the specific primers for fungal (ITS1/ITS4) and bacterial
(27F/1492R) DNA regions and identified nine pathogen species, excluding the causal
organism of the viral disease. The identified pathogens included Colletrotrichum orbiculare,
Cercospora citrullina, Fusarium oxysporum, Stagonosporopsis cucurbitacearum, Pseudoperonospora
cubensis, Phytophthora capsici, Acidovorax citrulli, Pseudomonas syringae,
and Serratia marcescens. The sequencing of the identified pathogens revealed high homology
(98.91–99.71%) with known sequences in the GenBank database. Phylogenetic analysis
showed six clusters for fungal and water mold pathogen isolates and three for bacterial
isolates where the percentages of replicate trees were 100% in all the cases. Among the
identified diseases, the highest disease occurrence was caused by Fusarium wilt (47.5%)
followed by Gummy stem blight (41.5%) in the Sylhet region and Angular leaf spot (37.5%)
followed by Yellow vine (33%) in the Natore area. Fusarium wilt also has a high disease
intensity, demonstrating its devastating impact on yield. This study highlights the influence
of environmental conditions on disease incidence and underscores the need for tailored
management strategies. These findings provide a foundation for developing targeted disease
management practices for sustainable watermelon cultivation in Bangladesh.
