Abstract:
The experiment was undertaken with a view to establish a protocol for in vitro culture
and plant regeneration using potato meristem as explant collected from potato sprouts
of six popular potato varieties Diamant, Cardinal, L. Rosetta, Granola, Asterix and
BARI ALU 29. The experiment was setup at Tissue culture laboratory of Debiganj
farm, Panchagarh. from March, 2015 to February, 2016. In the present study four
levels of GA3 (100, 200, 300 and 400 ppm) were used to assess the influence of GA3
on sprouting abilities of six popular potato verities. The maximum sprouting
efficiency was observed in 400 ppm GA3 treatment. Seven levels of GA3, 2, 4-D and
Kinetin were used along with fresh MS media to inoculate meristems of potato
sprouts. Maximum size of callus (0.82 cm) was observed in Cardinal inoculated in T3
(0.5 mg/l GA3 +1.0 mg/l 2, 4-D+ 1.5 mg/l KIN). Granola meristem inoculated in
hormonal treatment T2 (0.5mg/l GA3 + 0.5mg/l 2, 4-D + 1.5mg/l KIN) showed the
best results regarding minimum days required to shoot initiation (5.13 days) and the
single shoot length (2.41 cm). Three levels of GA3 treatments (T1- fresh MS, T2-
MS+0.5mg/l GA3 and T3- MS+1.5mg/l) were used to study the subsequent shoot
elongation and plant regeneration of single shoot produced from meristem culture. On
DAS- ELISA test all genotypes showed negative result against virus. Among all
varieties Diamant was the least responsive whereas Granola showed overall better
performance from meristem culture to establishment of plants in field conditions.
Description:
A Thesis
Submitted to the Department of Plant Pathology
Sher-e-Bangla Agricultural University, Dhaka
In partial fulfilment of the requirements for the degree of
MASTER OF SCIENCE (M.S.)
IN
PLANT PATHOLOGY
SEMESTER: JANUARY-JUNE 2015
