Abstract:
The present research was carried out in Biotechnology Laboratory of the
Department of Biotechnology, Sher-e-Bangla Agricultural University, Sher-eBangla
Nagar,
Dhaka-1207
from
the
period
of
September
2016
to
July
2017
for
in
vitro regeneration of onion (Allium cepa L.) under salt stress condition.
Three genotypes namely Faridpuri, Taherpuri and Pusa red (Indian) were used
as investigated genotypes in this study. Shoot tip segments of these genotypes
were cultured in MS (Murashige and skoog, 1962) media having 0.0 (control),
25, 50, 75 and 100 mM NaCl. The shoot tips of experimental onion genotypes
were not significantly affected upto 75 mM salinity level in comparison with
control treatment. The experiment was conducted at two factorial (variety and
Treatment) Completely Randomized Design (CRD) with 3 replications for each
treatment. Salinity response on days required for shoot regeneration varied
significantly from different salinity condition. The genotype Faridpuri was
found maximum tolerant upto 100mM salinity level with 10.60 cm shoot length
and 1.43 cm root length having the highest relative shoot and root growth.
Besides, Pusa red was found as salinity sensitive genotype showing the lowest
7.03 cm shoot length and 0.60 cm root length at 100 mM NaCl treatment.
However, Taherpuri was found tolerant up to 75 mM salinity level with 8.30
cm shoot length and 1.43 cm root length, respectively. The genotypes
Faridpuri, Taherpuri and Pusa red (Indian) can be used for further investigation
in field condition to evaluate their performance at various salinity levels.
Therefore, a convenient in vitro regeneration protocol of onion genotypes
under different salinity level has been developed which can be used for
screening salinity tolerant variety of onion within a very short period of time.
Description:
A Thesis
Submitted to the Faculty of Agriculture,
Sher-e-Bangla Agricultural University, Dhaka,
in partial fulfillment of the requirements
for the degree of
MASTER OF SCIENCE
IN
BIOTECHNOLOGY
