http://localhost:8080/handle/123456789/3676 2026-04-08T14:38:01Z http://localhost:8080/handle/123456789/3781 SOCIO DEMOGRAPHY OF BROILER FARMERS, THEIR PERCEPTION AND INDISCRIMINATING USE OF ANTIBIOTIC AND ITS EFFECTS LINA, NUSRAT JAHAN Residual result of antibiotics and its inefficacy to microorganisms has been recognized as a public health hazard worldwide. This study was conducted among 98 broiler farms of three selective districts of Bangladesh from August to December, 2019 to explore the antibiotic use pattern and perception of farmers on antibiotic. Semi-structured questionnaire was used to gather information. Farmers administered antibiotics mainly as therapeutics (84.69%). Over one third (33.67%) farmers used antibiotic as prophylaxis. Almost half (48.97%) bought antibiotics with registered vet doctor’s advice. But 43.88% farmers took self-decision for antibiotics use. Ciprofloxacin (41.83%), Colistin sulphate (24.49%) and Enrofloxacin (17.35%) were found to be three main therapeutic drugs of choice. Nearly half (47.96%) of respondents thought that malabsorption was the main residual effect of antibiotics. A vast majority (91.84%) of poultry growers had no idea about antibiotic resistance. Antibiotics were used ordinarily in broiler farm in the study area. Intervention should be taken to minimize the use of unethical and self-choosing antibiotics in the study area. A Thesis Submitted to the Faculty of Agriculture, Sher-e-Bangla Agricultural University, Dhaka. In partial fulfillment of the requirements for the degree of MASTER OF SCIENCE IN BIOCHEMISTRY SEMESTER: JULY- DECEMBER, 2020 2020-12-01T00:00:00Z http://localhost:8080/handle/123456789/3780 EVALUATION OF PHYSICO-FUNCTIONAL CHARACTERISTICS AND NUTRITIONAL STATUS OF SOME PIGMENTED WHOLE GRAIN AVAILABLE IN BANGLADESH SUBRIN, SUMAIYA Maize and rice are the highest consumed cereals in the world. Pigmented and nonpigmented maize can be utilized like rice if its complete properties are known. There are some pigmented and non-pigmented maize and rice varieties available in Bangladesh for human consumption. The present research was conducted to evaluate and compare physico-functional and nutritional properties of colored maize and rice. Maize such as-yellow maize, red maize, white maize, purple maize, mixed colored maize, deep red maize; and rice such as red rice was used for investigation. Study revealed that white maize was the brightest grain among the samples with its highest L* value while purple maize, deep red maize and red rice showed the lowest L* value. The lowest bulk density (0.565 ± 0.005 g/mL) and % change (15.05 ± 0.31%) in sedimentation was recorded for purple maize flour but white maize flour showed the highest change in sedimentation value (35.43 ± 0.59%). All most all samples were comparable for proximate composition. Red maize and purple maize contained the highest amount of ash (2.27 ± 0.059% and 2.27 ± 0.05% respectively) while mixed maize contained the lowest amount of ash (1.22 ± 0.09%). Mixed maize contained the highest crude fibre (4.17 ± 0.049%) which was comparable to red rice on the other hand white maize had the lowest crude fibre (3.31 ± 0.06%). Purple maize along with mixed maize and deep red maize had the highest carbohydrate content (72 to 73%) whereas local mixed maize and deep red maize had the lowest protein content (6%). Red maize had the highest protein content which was comparable to yellow maize, and the lowest carbohydrate content (65.76 ± 0.57%) which was comparable to white maize and red rice. Red rice performed better than the other samples for oil content (6.19%). In case of Mg and S content, all most all samples showed similarity; and the purple maize, mixed maize and deep red maize were promising for Ca content. White maize had the highest amount of Zn (19.79 ± 0.1 mg/100 g) while it had the lowest amount of Ca (140.16 ± 10.8 mg/100 g), and Mn (0.51 ± 0.05 mg/100 g). Yellow maize showed the highest amount of Fe (4.99 ± 0.37 mg/100 g); and the lowest Cu content (0.14 ± 0.02 mg/100 g) which was similar to mixed maize (0.13 ± 0.01 mg/100 g). The red rice was poor in Zn (4.02 ± 0.13 mg/100 g) and Fe (0.61 ± 0.14 mg/100 g) content. Overall, whole grain maize and rice samples were comparable from the physico-functional and nutritional properties. Therefore, whole grain pigmented and non-pigmented maize can be utilized like rice as rich source of nutrition as regularly consumed cereals to combat nutritional deficiency. A Thesis Submitted to the Faculty of Agriculture Sher-e-Bangla Agricultural University, Dhaka, in partial fulfillment of the requirements for the degree of MASTER OF SCIENCE IN DEPARTMENT OF BIOCHEMISTRY SEMESTER: DECEMBER, 2020 2020-12-01T00:00:00Z http://localhost:8080/handle/123456789/3779 BIOCHEMICAL AND MOLECULAR CHARACTERIZATION OF SOME ADVANCED MUTANT RICE (Oryza sativa) LINES OF BINA NADIM, MD. KAWSAR ALAM Rice (Oryza sativa) is one of the oldest cereal grains in the world and the main staple food of Bangladesh. In rice, amylose and protein are the main biochemical factors in selection and breeding of good eating quality varieties. A total of 12 rice genotypes [10 advanced mutant lines, 1 released mega variety (BRRI dhan49) and 1 landrace (Luxmidigha)] was incorporated in this study to assess their amylose and protein content as well as their genetic diversity relationship. Among the genotypes, Magic-86 was highest in amylose content (25.6%). The second and third highest amylose content were RM-40(C)-4-2-8 (25.1%) and Magic-10 (24.4%), respectively. Amylose of other genotypes ranged from 23.9 to 20.3%. SSB-3 was found to be the highest in protein content (8.86%) whereas Magic-86 (8.78%) and Luxmidigha (8.73%) were the second and third highest, respectively. Protein content of other genotypes were ranged from 8.50 to 5.04%. The highest gene diversity (0.81) was observed in RM519 and the lowest (0.28) was in RM111. The PIC values ranged from 0.24 to 0.78. The highest Nei’s genetic distance value 0.87 was found in RM-16(N)-10 vs RM-40(C)-4-2-8 and the lowest value 0.18 was found in LD-200-1-3-3-8 vs LD-200-1-3-2-4. A dendogram was constructed based on Nei’s similarity coefficient using UPGMA method to group the selected genotypes and observe relationship among them. Six major clusters were built at 0.7 cut off similarity coefficient. The highest number of genotypes was observed in cluster-6 (4 genotypes) and the lowest in cluster-1, cluster-2 and cluster-4 (each contained 1 genotype). Cluster-3 and cluster-5 contained 3 and 2 genotypes, respectively. The genotypes showing diverse ranges of amylose and protein content tended to cluster together in the dendogram with some exceptions. These findings may play potential role for the selection of parents or lines in future breeding program to develop better quality rice varieties for Bangladesh. A Thesis Submitted to the Faculty of Agriculture Sher-e-Bangla Agricultural University, Dhaka-1207 in partial fulfilment of the requirements for the degree of MASTER OF SCIENCE (MS) IN BIOCHEMISTRY SEMESTER: JULY-DECEMBER-2020 2020-12-01T00:00:00Z http://localhost:8080/handle/123456789/3778 NUTRITIONAL STUDY OF SOME ADVANCED MUTANT LINES OF GROUNDNUT (Arachis hypogaea) MITU, MARIOM Groundnut, popularly known as "chinabadam" is the second most important oil seed crop in Bangladesh, after mustard (Brassica spp.) in terms of annual production. It is used as an edible oil in the food industry to produce cake, cookies, and baked goods. It plays an important role in human nutrition containing high protein, calories, minerals, and vitamins. Binachinabadam-6 and four advanced mutant lines derived from it, B6/282/63, B6/282/64, B6/282/80 and RM-Kha-19 were studied to investigate the qualitative properties of the genotypes and to find potential groundnut genotypes which can be released as variety. Among the 5 selected genotypes oil content were observed from 54.6% to 48.5%. Myristoleic acid (2.463-0.009%), oleic acid (55.7-29.977%), linoleic acid (17.129-13.691%), erucic acid (3.035-1.634%) as unsaturated fatty acids and hexanoic acid (23.291-4.232%), lauric acid (4.742-1.302%), palmitic acid (8.6601.044%), arachidic acid (28.455-16.829%) were estimated as saturated fatty acids. O/L ratio (3.68-1.75) and iodine value (74.12-53.71) were calculated from fatty acid components. Ca (85.54-74.12 mg/100g), Mg (173-148 mg/100g), S (89-56 mg/100g), N (5190-4370 mg/100g), P (490-360 mg/100g), K (730-650 mg/100g) as major minerals and Fe (2.15-1.62 mg/100g), Zn (5.9-3.8 mg/100g), Cu (1.5-0.5 mg/100g), B (3.6-2.4 mg/100g) were estimated as minor minerals. Other properties i.e protein (32.42-27.34%), ash (2.38-2.23%), crude fiber (3.71-3.52%), carbohydrate (11.4- 5.57%) and total energy (628.4- 594.58%) were also observed. Considering all the findings, B6/282/80 was the best performer among all mutants in this study. A Thesis Submitted to the Faculty of Agriculture Sher-e-Bangla Agricultural University, Dhaka-1207 in partial fulfilment of the requirements for the degree of MASTER OF SCIENCE (MS) IN BIOCHEMISTRY SEMESTER: JULY-DECEMBER-2020 2020-12-01T00:00:00Z